几种脂肪酶在亨氏马尾藻多不饱和脂肪酸提取中的初步应用研究  

Study on the application of some lipases to the extraction of PUFAs from Saragassurn henslowianum

作　　者：黄俊辉[1] 成坚[2] 曾庆孝[1] 肖蕾[1] 佘纲哲[3] 

机构地区：[1]华南理工大学食品与生物工程学院,广东广州510640 [2]仲恺农业技术学院食品科学系,广东广州510225 [3]汕头大学科学院,广东汕头515063

出　　处：《仲恺农业技术学院学报》2000年第4期17-22,共6页Journal of Zhongkai Agrotechnical College

摘　　要：研究了脂肪酶的种类、用量、水解温度、溶剂等因素对南沙马尾藻总脂质水解率的影响 ,实验表明 :以体积分数 0 85的乙醇水溶液为水解溶剂 ,PocinePancreas用量为质量分数 0 0 12 7、水解温度 30℃、时间 15h时 ,总脂质的水解率可达 71.4 1%Effects of kinds and dosage of lipase added to the extraction of PUFAs, and solvent, hydrolyzing temperature and time on the hydrolyzed rate of total lipids of Sargassum henslowianum come from South coral archipelago of South China Sea were studied. Experimental results showed that total fat hydrolyzing rate was up to 71.41% and 99.23% saturated fat acid could be hydrolyzed in fat from the seaweeds under conditions that the dosage of Pocine Pancreas was 1.27%, the solvent of hydrolysis was 85% ethanol, the hydrolysis temperature was 30℃ and hydrolysis time was 15 hours.

关 键 词：脂肪酶 海藻 多不饱和脂肪酸 水解率 提取 

分 类 号：Q547[生物学—生物化学] Q55