米糠阿魏酰低聚糖的纤维素酶法提取工艺及其抗氧化活性  

Study on enzymatic production of feruloyl oligosaccharides from defatted rice bran and its antioxidant activity

作　　者：盛情情 王文侠[1] 宋春丽[1] 张慧君[1] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院黑龙江省普通高校农产品加工重点实验室,黑龙江齐齐哈尔161006

出　　处：《食品与发酵工业》2013年第12期197-203,共7页Food and Fermentation Industries

基　　金：齐齐哈尔大学研究生创新科研项目(YJSCX2013-036X)

摘　　要：以脱淀粉、脱蛋白的脱脂米糠为原料,对纤维素酶水解米糠提取阿魏酰低聚糖(FOs)的工艺进行了研究。在单因素实验的基础上,以FOs的提取量为响应值,采用响应面分析法对提取条件进行优化,得到最佳提取条件为:pH4.16,反应温度41.48℃,加酶量0.29 mg/g,底物浓度3.17%,反应时间11.76 h,在此条件下,FOs的提取量达到28.48μmol/g。米糠阿魏酰低聚糖对DPPH·和·OH均有一定的清除作用。其对DPPH·、·OH的EC50分别为0.750 5 mg/mL和3.270 5 mg/mL。In order to improve the extraction efficiency of feruloyl oligosaccharides （FOs） from defatted rice bran using cellulase hydrolysis, single factor experiments and response surface analysis were employed to optimize the enzy- molysis. The results suggested that the optimal extraction conditions were as follows： pH 4.16, reaction temperature 55℃ , enzyme addition amounts 0.29 mg/g, and reaction time 11.76 h. Under these conditions, the extraction a- mount of FOs reached 28.48 μmol/g. And the scavenging effect of FOs on the DPPH ·and ·OH; ECS0 were 0. 750 5 mg/mL and 3. 270 5 mg/mL, respectively.

关 键 词：脱脂米糠 阿魏酰低聚糖(FOs) 纤维素酶 抗氧化活性 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]