HS-SPME-GC-MS分析香荚兰豆中挥发性成分  

Analysis of Volatile Chemical Constituents in Vanilla planifoli by HS-SPME-GC-MS

作　　者：卢金清[1] 李雨玲[1] 张锐[1] 杨小金[1] 郭彧[1] 江汉美[1] 李肖爽[1] 

机构地区：[1]湖北中医药大学/湖北省药用植物研发中心,武汉430065

出　　处：《中国实验方剂学杂志》2014年第3期79-82,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖北省自然科学基金项目(2004AB242)

摘　　要：目的:研究香荚兰豆中挥发性成分的化学组成。方法:采用顶空固相微萃取(HS-SPME)技术对香荚兰豆挥发性成分进行萃取、富集,气相色谱-质谱(GC-MS)法进行分离分析,并筛选了固相微萃取纤维头,优化了SPME的操作条件。结果:共分离并鉴定出28种化学成分,占挥发性成分总量的98.17%。香荚兰豆挥发性成分中含量最高的是香兰素(48.28%),其次为愈创木酚(15.54%)。结论:采用HS-SPME-GC-MS联用技术分析香荚兰豆挥发性成分,为香荚兰豆进一步研究及综合开发利用提供科学依据。Objective： To analyze the volatile components from fruit of Vanill planifol. Method： The volatile chemical constituents were extracted and enriched by headspace solid phase micro-extraction （HS-SPME）. The chemical components were analyzed by GC-MS. Selected the solid phase micro-extraction fiber head, while optimized the HS-SPME operating conditions. Result： A total of 28 components were separated and identified, accounted for 98.17% of total volatile components. The main component is vanillin （48.28%） and 2-methoxy- phenol （15.54%）. Conclusion： This is the first time to adopt HS-SPME combined with GC-MS to analyze the volatile chemical components from fruit of V. planifoli and this study can provide scientific basis for further research and development of volatile chemical constituents of V. planifoli.

关 键 词：香荚兰豆 固相微萃取 气相色谱-质谱 挥发性成分 

分 类 号：R284.1[医药卫生—中药学]