杜仲叶抗α-葡萄糖苷酶成分的研究  

Study on Anti-Alpha Glucosidase Components from Eucommia ulmoides Leaves

作　　者：沈伊濛 王丽卫[3] 文羽思 王仁涛[1,2] 范俊峰[1,2] 

机构地区：[1]北京林业大学生物科学与技术学院 [2]北京林业大学林业食品加工与安全北京市重点实验室,北京100083 [3]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《中国食物与营养》2014年第1期58-61,共4页Food and Nutrition in China

基　　金：2011年北京市大学生科学研究与创业行动计划项目

摘　　要：通过正交试验优化了杜仲叶中抗α-葡萄糖苷酶成分的提取条件,并利用葡聚糖凝胶对杜仲叶水提液进行纯化分离,分析了杜仲叶水提物纯化液的黄酮含量。研究结果表明,杜仲叶的提取条件为提取料液比为1∶10,提取温度为90℃,提取时间为40min。杜仲叶水提物纯化液具有明显的抗α-葡萄糖苷酶效果,且杜仲叶中总黄酮含量为3.31%,纯化液中黄酮的含量为18.12%,杜仲总黄酮粗品得率为7.32%。This paper studied the optimum extraction conditions of anti-alpha glucosidase components from Eucommia ulmoides leaves through the orthogonal experiment. Water extraction of Eucommia ulmoides leaves was separated and purified by Sephadex G-75, and the fla- vonoid content was analyzed. The optimal extraction conditions ot＇ Eucommia ulmoides leaves were ratio of solid to liquid 1：10 （w/v）, ex- traction temperature 90~C, and extracting time 40 min. The purified water extract of Eucommia ulmoides leaves had obvious anti-alpha gluco- sidas effect. The flavonoid content of Eucommia ulmoides leaves and the purified extract were 3.31% and 18.12% , respectively. The yield of crude flavonoids extract was 7.32% .

关 键 词：杜仲叶 黄酮 Α-葡萄糖苷酶 降血糖 

分 类 号：R284[医药卫生—中药学]