南极磷虾抗氧化多肽制备的研究  

Preparation of Antarctic krill peptides with anti-oxidant activity

作　　者：王继宏[1] 汪之和[1] 田鑫[1] 李燕[1] 

机构地区：[1]上海海洋大学食品学院,上海201306

出　　处：《食品工业科技》2014年第3期109-112,共4页Science and Technology of Food Industry

基　　金：国家高新技术研究发展(863);计划海洋技术领域或主题研究(2011AA090801)

摘　　要：以清除超氧阴离子自由基、DPPH自由基和羟基自由基能力为指标，筛选制备南极磷虾杭氧化多肽的水解条件，得到高效的抗氧化多肽制品。结果表明：胰蛋白酶、中性蛋白酶复合，酶解南极磷虾6h后得多肽的抗氧化能力最强，超氧阴离子自由基的清除率达到11．5％，DPPH自由基的清除率达到72．7％，羟基自由基的清除率达到84．8％。经超滤分离，分子量范围在5-10ku的多肽表现出较强的清除三种自由基的能力。对其进行tricine—SDS—PAGE电泳，结果显示电泳图谱上出现了两个条带。In the study, the free radical scavenging ability against superoxide, DPPH and hydroxyl radical was investigated to screen optimum hydrolysis conditions for preparing Antarctic krill peptides with high anti-oxidant activity.Results indicated that peptides prepared by trypsin and neutral protease for 6h,showed the highest anti- oxidant activity,with free radical scavenging effect on superoxide, DPPH and hydroxyl radical by 11.5% ,72.7% and 84.8%, respectively.Through ultra-filtration, peptides （5-10ku） exhibited stronger scavenging activity against free radicals,which further revealed with two bands in the tricine-SDS-PAGE electrophoretogram.

关 键 词：抗氧化 南极磷虾 酶解 超滤 tricine—SDS—PAGE电泳 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]