樟子松树皮多酚二次纯化及成分分析  

Secondary purification and composition analysis of polyphenols from Pinus sylvestris L.barks

作　　者：刘荣[1] 姜元松[1] 杨巍巍[1] 王振宇[1,2] 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150090

出　　处：《食品工业科技》2014年第3期113-118,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(31170510)

摘　　要：以吸附解析率为指标对供试9种大孔树脂进行筛选,选择出最优树脂以纯度及回收率为指标对樟子松树皮多酚进行二次纯化,最后HPLC-DAD-ESI-MS分析方法对多酚成分进行了初步分析。结果表明:X-5大孔树脂为最优纯化树脂,60%浓度的乙醇为最优洗脱剂;樟子松树皮多酚二次纯化的最适宜条件为:上样浓度4.0mg/mL,吸附柱径高比1∶30,上样体积60mL,洗脱流速2BV/h。在此二级纯化条件下,樟子松树皮多酚纯度可由62.06%±1.67%提升到72.99%±0.98%,回收率为87.26%±2.42%;二级纯化物中主要成分推测为:原花青素三聚体、原花青素二聚体B2、5-邻-没食子酰基奎尼酸、表儿茶素以及鞣花酸。The best resin was selected from nine kinds of macroporous resins by adsorption and resolution rate as indicators.Pinus sylvestris L.barks polyphenol was secondary purified with the best resin using purity and recovery rate as index.Finally,the composition of secondary purified polyphenol was preliminary analyzed with HPLC-DAD- ESI-MS method.The results showed that the macroporous resin X-5 was the best resin and 60% ethanol was suitable for eluent.The optimum condition of secondary purification was that sample solution concentration was 4.0mg/mL,chromatographic column diameter hight ratio was 1：30, sample solution volume was 60mL, eluting speed was 2BV/h.Under this condition,the purity of Pinus sylvestris L.barks polyphenol increased from 62.06% ±1.67% to 72.99% ±0.98%, the recovery rate was 87.26%±2.42%.The main composition of secondary purification product were.procyanidins trimer, procyanidin dimmer type B2,5-galloylquinic acid,epicatechin and ellagic acid.

关 键 词：大孔树脂 樟子松 多酚 二次纯化 HPLC—DAD—ESI—MS 

分 类 号：TS201.1[轻工技术与工程—食品科学]