印度块菌多糖的纯化及其理化性质研究  

Purification and determination of physicochemical property of Tuber indicum polysaccharide

作　　者：肖伟东[1] 邰丽梅 孔庆龙[1] 刘蓓 樊建[1] 赵天瑞[1] 

机构地区：[1]昆明理工大学化学工程学院,云南昆明650500 [2]云南省供销合作社科学研究所,云南昆明650221

出　　处：《食品工业科技》2014年第3期135-137,共3页Science and Technology of Food Industry

基　　金：云南省科技厅社会发展科技计划科研院所技术开发研究专项(2011CF015)

摘　　要：以云南产印度块菌为原料,采用水提醇沉法从其子实体中提取粗多糖,经过Sevage法脱蛋白、DEAE-52纤维素柱层析分离,得到3种多糖组分PTI-1、PTI-2、PTI-3,选择PTI-2通过Sephadex G-100柱进一步纯化,得到了均一纯多糖,命名为PTI-2A,并对PTI-2A进行基础理化测定。结果表明:PTI-2A分子量为193388.2u,易溶于水、稀酸、稀碱,不溶于甲醇、丙酮、氯仿、乙醚和二甲基亚砜等有机溶剂,不含蛋白质、核酸、酚类、淀粉和游离单糖。The crude polysaccharide was extracted from the India truffle fruit body produced in Yunnan by the water extraction and alcohol sink method. After the separation of the Savage deproteinization and the DEAE cellulose column chromatography,three components： PTI- 1, PTI-2, PTI-3 were obtained, among which PTI-2 was further purified by sephadex G-100 gel chromatography .And then a single polysaccharide called PTI-2A was got and the basal physicochemical properties of it was measured.The result showed that molecular weight of PTI-2A was ]93388.2u and it was easily dissolving in water, dilute acid, dilute alkalis, but insoluble in organic solvent like methanol, acetone, chloroform, diethyl ether and dimethyl sulfoxide, etc., and it didn＇ t contain protein, nucleic acid, phenols,amylum and free monosaccharide.

关 键 词：印度块菌 多糖 纯化 理化性质 

分 类 号：TS202.3[轻工技术与工程—食品科学]