等离子体诱变凝结芽孢杆菌提高木糖利用能力高产L-乳酸  

Improved Xylose Utilization of Bacillus coagulans by Atmospheric and Room Temperature Plasma Mutation for Production of Lactic Acid

作　　者：蔡聪[1,2] 姜婷[2] 郑兆娟[2] 欧阳嘉[1,2] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]南京林业大学江苏省生物质绿色燃料与化学品重点实验室,江苏南京210037

出　　处：《食品科学》2014年第1期125-129,共5页Food Science

基　　金：国家自然科学基金项目(31200443);江苏省杰出青年基金项目(BK2012038);国家"863"计划项目(2012AA022301)

摘　　要：为进一步提高凝结芽孢杆菌发酵木糖生产L-乳酸的产量和转化率,以实验室保存的一株能利用木糖产L-乳酸的野生型凝结芽孢杆菌菌株NL01为出发菌株,通过等离子体诱变育种技术和平板菌落初筛、摇瓶复筛,最终得到一株木糖耐受力强、L-乳酸产量高、遗传特性稳定的正向突变菌株NL-CC-17。该突变菌株是目前已报道的木糖耐受力最高的菌株。当以100 g/L的木糖为底物,50℃发酵72 h后,L-乳酸产量达到82.30 g/L,糖酸转化率为92.37%,L-乳酸产量较出发菌株提高21.51%,糖酸转化率提高了16.00%。通过初步优化发酵条件,确定该菌株最佳发酵温度为50℃,实验范围内最佳发酵pH值为5.5。To further enhance the production and yield of converting xylose to L-lactic acid, an atmospheric- and roomtemperature-plasma （ARTP） mutation system was employed to induce the mutation of Bacillus coagulans NL01, a wild-typestrain for production of L-lactic acid from xylose. A positive mutant, named as NL-CC-17, was obtained through enrichment andscreening. Compared with that of previously reported strains, NL-CC-17 had an improved tolerance to xylose. This strain wascapable of converting 100 g/L xylose into 82.30 g/L L-lactic acid after being cultured at 50 ℃ for 72 h with a yield of 92.37%. Theconcentration of L-lactic acid was increased by 21.51% and the yield by 16.00%. After preliminary optimization of fermentationconditions, the optimal fermentation temperature was determined as 50 ℃ and the optimal pH as 5.5.

关 键 词：常温常压等离子体 凝结芽孢杆菌 诱变 L-乳酸 木糖 

分 类 号：Q815[生物学—生物工程]