油脂酵母Trichosporon fermentans HWZ004的分批补料法高密度培养  

Fed-Batch Culture of High Cell-Density Oleaginous Yeast Trichosporon fermentans HWZ004

作　　者：宗敏华[1,2] 刘宗俊 吴虹[1,2,4] 娄文勇[1,2] 朱定和[2,4] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640 [2]华南理工大学轻工与食品学院,广东广州510640 [3]华南理工大学生物科学与工程学院,广东广州510640 [4]广东省天然产物绿色加工与产品安全重点实验室,广东广州510640

出　　处：《华南理工大学学报（自然科学版）》2013年第10期47-52,65,共7页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31071559);教育部新世纪优秀人才支持计划项目(NCET-11-0161;NCET-10-0367);制浆造纸工程国家重点实验室开放基金资助项目(201138);广东省天然产物绿色加工与产品安全重点实验室开放基金资助项目(201207)

摘　　要：为提高发酵性丝孢酵母Trichosporon fermentans HWZ004发酵产油脂的能力,采用分批补料的培养方式对其进行高密度培养,考察了初始糖浓度对发酵性丝孢酵母生长及油脂积累的影响,确定初始糖浓度为100 g/L,在该浓度下Trichosporon fermentans HWZ004可获得较高的生物量及油脂产量,且不存在底物抑制现象;然后,在5 L发酵罐中进行分批补料培养,发酵132h后,Trichosporon fermentans HWZ004的生物量、油脂含量及油脂生产强度分别为102g/L、48%和0.37g/(L·h).气相色谱分析结果表明,所得油脂的脂肪酸组成主要为硬脂酸、棕榈酸、油酸、亚油酸,不饱和脂肪酸含量达65%以上,适用于生物柴油的生产.In order to improve the ability of oleaginous yeast Trichosporon fermentans HWZ004 to produce lipid,a fed-batch culture with high cell density was carried out.The effect of the initial sugar concentration on the growth and lipid accumulation of T.fermentans HWZ004 was investigated and the initial sugar concentration was determined as 100 g/L,at which T.fermentans HWZ004 could achieve more biomass and higher lipid yield without substrate inhibition.After a 132h fed-batch culture in a 5-L fermentor,it is found that the biomass,lipid content and lipid productivity of T.fermentans HWZ004 are respectively 102g/L,48.3％ and 0.37g/（L · h）.GC results reveal that the fatty acids in the obtained lipid are mainly stearic acid,palmitic acid,oleic acid and linoleic acid,and the unsaturated fatty acid content is over 65％,which means that the lipid is suitable for the biodiesel production.

关 键 词：发酵性丝孢酵母 分批补料式发酵 高密度培养 生物量 油脂产量 

分 类 号：TQ630.41[化学工程—精细化工] Q815[生物学—生物工程]