酵母抽提物辐照挥发性成分分析  

Analysis of Volatiles from Irradiated Yeast Extract

作　　者：廖涛[1] 李新[1] 鉏晓艳[1] 陈玉霞[1] 耿胜荣[1] 

机构地区：[1]湖北省农业科学院农产品加工与核农技术研究所/湖北省农产品辐照工程技术中心/湖北省农业科技创新中心,湖北武汉430064

出　　处：《核农学报》2013年第12期1858-1864,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金青年项目(11105048)

摘　　要：采用顶空固相微萃取（headspace solid phase micro-extraction，HS-SPME）和气相色谱一质谱联用法（gas chromatography-mass spectrometry，GC-MS）分析酵母抽提物（yeastextract，YE）辐照0～19．8kGy后的挥发成份。以醛、酮、醇、酸、酯类以及含硫化合物的峰面积和化合物个数为参照对象，探讨和优化了HS-SPME的最佳萃取条件：50／30μm涂层为二乙烯基苯-碳分子筛-聚二甲基硅氧烷萃取头（Divinylbenzene／carboxen／polydimethylsiloxane，DVB／CAR／PDMS）、萃取温度40℃、萃取时间40rain。利用优化的HS-SPME萃取条件分别萃取了辐照0～19．8kGyYE的挥发成分，并经气相色谱-质谱联用仪分析检测。结果表明，未辐照YE中共检出15种挥发性物质，主要成分为醋酸、2，3-丁二醇和2-乙基己醇。辐照YE共检出40种挥发性物质，主要成分为醋酸、B-苯乙醇、庚醛、天然壬醛，其中醛类、酮类、烯类和二甲基二硫是新产生的。The method for determination volatiles from irradiated yeast extract （YE） using headspace solid phase microextraction （HS-SPME） and gas chromatography-mass spectrometry （GC-MS） was developed in this paper. The extraction conditions were optimized with reference to the peak area and number of volatiles as aldehyde, ketone, alcohol, acid,ester and sulfur compounds. The optimized conditions of HS-SPME for volatiles in irradiated YE were： divinyl benzene/Carboxen/polydimethylsiloxane （DVB/CAR/PDMS） fiber, extration time 40min, extraction temperature 40~C. The volatiles fromYE irradiated by 0 - 19.8kGy were detected using HS-SPME coupled with GC-MS. The results showed that only 15 volatiles were detected from no irradiated YE and main compounds were acetic acid,2,3 - butanediol and 1 -hexanol,2- ethyl-. There were 40 volatiles detected from irradiated YE and the main compounds were acetic acid, phenylethyl alcohol, heptanal and nonanal. Compare to no irradiated yeast extract, the aldehyde, ketone, alkene and disulfide,dimethyl were produced by irradiating process.

关 键 词：酵母抽提物 顶空固相微萃取 气质联用仪 挥发性成分 辐照 

分 类 号：TS207.3[轻工技术与工程—食品科学]