外源Zn^(2+)在大麦发芽时的动态分布  

Transporting trend of external Zn^(2+) in malting process

作　　者：朱重悦[1] 王越[1] 陈莉[1] 安家彦[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2013年第5期329-331,共3页Journal of Dalian Polytechnic University

基　　金：辽宁省高校创新团队项目(2008T008);辽宁省高校创新团队支持计划项目(LT2012011)

摘　　要：添加Zn2+可以通过抑制TCA循环的苹果酸脱氢酶活性来降低制麦损失,为进一步深入分析Zn2+在大麦发芽过程的作用机理,采用双硫腙比色法检测了Sebastian和Schooner大麦发芽中各主要部位(麦皮、胚、胚乳)以及浸麦中添加Zn2+实验组大麦各部分Zn2+的质量分数。实验结果表明空白组和添加锌离子的实验组中Zn2+的运动趋势相同,都是从胚乳和麦皮向胚中运输扩散;通过检测大麦发芽前后总锌含量得出,虽然锌在大麦各部位含量变化很大,但始终处于动态平衡。The malting loss can be reduced by Zn2＋inhibiting malate dehydrogenase activity in TCA cycle.The dynamic change of Zn2＋distribution was discussed for further analysing the mechanism of Zn2＋in barley germination process.Content of Zn2＋in main malt position within Sebastian and Schooner barley has been detected by dithizone colorimetric method.The result shows that the motions of control group and experimental group,transport diffusion from endosperm and bran to the centre of embryo substantially.Zn2＋is always in a dynamic balance before and after germination,though the zinc content in each part of barley changed a lot.

关 键 词：麦芽 锌离子 双硫腙 

分 类 号：TS261.2[轻工技术与工程—发酵工程]