食用油中5种邻苯二甲酸酯的液液萃取-固相萃取耦合富集和气质联用的测定  

Determination of five phthalate acid esters in edible oil by GC-MS and coupled enrichment of liquid-liquid extraction and solid phase extraction

作　　者：曹九超[1] 俞晔[1,2] 朱云 金青哲[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]张家港出入境检疫局.江苏张家港215633 [3]无锡中粮工程科技有限公司,江苏无锡214035

出　　处：《中国油脂》2014年第1期43-46,共4页China Oils and Fats

基　　金：“十二五”国家科技支撑计划(2012BAK08B03)

摘　　要：建立了食用油中常见的5种邻苯二甲酸酯（PAEs）类塑化剂的检测方法。食用油样品经乙腈提取后进行冷冻脱脂处理、玻璃ProElutPSA固相萃取柱净化，GC—MS全离子扫描定性、选择离子监测模式（SIM）定量。结果显示：食用油中5种PAEs加标回收率在76．17％-98．8l％之间，相对标准偏差在1．01％～7．20％之间。该方法的仪器检出限（信噪比为3）和最低定量限分别在0．3～2．0μg／L及1．0～20．2μ/kg之间。该方法检测食用油中PAEs类塑化剂具有较高的准确度和灵敏度。A method for determination of five common phthalate acid esters （PAEs） in edible oil was established. The edible oil samples were extracted by acetonitrile and then frozen to defat, and cleaned up with glass ProElut PSA solid phase extraction column. The qualitification and quantification of the five PAEs were performed by GC -MS in full ion scanning and selected ion monitoring （SIM） mode respec- tively. The results showed that the average recovery rates of standard addition of the five PAEs in edible oil ranged from 76.17% to 98.81% with 1.01% -7.20% of the relative standard deviations. The in-strumental detection limits （ S/N= 3 ） of the method ranged from 0.3 μg/L to 2.0 μg/L, and the limits of quantitation were 1.0 - 20.2μg/kg. This method for the determination of the five PAEs in edible oil had high accuracy and sensitivity.

关 键 词：塑化剂 邻苯二甲酸酯 食用油 固相萃取 GC—MS 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.63[轻工技术与工程—食品科学与工程]