利用元素分析仪测定大米粗蛋白含量的方法探讨  

The Elemental Analyzer Method for Determination of Crude Protein Content in Rice

作　　者：钟国才[1] 陈威[1] 吴军辉[1] 陈嘉东[1] 周延智 

机构地区：[1]广东省粮食科学研究所,广州510050 [2]广东省储备粮管理总公司东莞直属库,东莞523145

出　　处：《食品工业》2014年第2期158-160,共3页The Food Industry

基　　金：广州市重大科技专项计划项目(2010U1-E00771)

摘　　要：提出了一种利用元素分析仪测定大米中含氮量预测粗蛋白的方法。对比凯氏定氮法,测定结果高于前者(p<0.05),两者之间的平均比值为0.988±0.008,回归预测方程为Y=0.248 84+0.950 92X(R2=0.937 71)。该方法的日内精密度为1.29%,日间精密度为1.79%,适合于准确、简便的筛选大批量的大米原料和产品中粗蛋白的含量。An elemental analyzer method （EA） for determination of crude protein content in rice was proposed. The results determined by EA is considerably higher than that by Kjeldahl method （KM） （p〈O.05）, the average ratio of the results determined by the two methods is 0.988i-0.008, and regression equations for Y=0.248 84＋0.950 92 X（R2=0.937 71）. Intra-day precision and inter-day precision of this method are 1.29% and 1.79% respectively. The data indicated that EA is easy to rapidly acquire the accurate measure results, which is suitable for testing crude protein content in rice, raw materials and products.

关 键 词：元素分析仪 凯氏定氮法 粗蛋白 大米 

分 类 号：TS212.7[轻工技术与工程—粮食、油脂及植物蛋白工程]