胶束增敏荧光法测定番茄红素的研究  

Determination of Lycopene by Micellar Enhanced Fluorimetry

作　　者：曹荻 李满秀[2] 杨淑英[2] 高雷[2] 

机构地区：[1]瑞士水低因咖啡有限公司 [2]忻州师范学院化学系,忻州034000

出　　处：《食品工业》2014年第2期168-170,共3页The Food Industry

基　　金：山西省高等学校教学改革项目(编号J2012091);山西省高等学校大学生创新创业训练项目(编号2012334);忻州师范学院教学改革项目(编号201113)

摘　　要：根据表面活性剂具有胶束增敏的作用,发现十六烷基三甲基溴化铵(CTMAB)在pH为11.0的三酸缓冲溶液中,对番茄红素的荧光有增敏作用,据此实现了对其灵敏的检测。试验分别对表面活性剂及用量、介质pH、温度及放置时间进行了探讨,选择最佳试验条件,建立了用增敏荧光法测定番茄红素含量的新方法。该方法的线性范围为9.7μg/mL^85.9μg/mL,检出限为:1.1μg/mL。方法用于番茄红素含量的测定,结果令人满意。A fluorescence spectrophotometric determination of lycopene has l^een studied by added CTMAB. In pH 11.0 medium, CTMAB can enhance the fuorescence of lycopene and the measurement has been finished by used Fluorescence Spectrophotometry. It was investigated by testing many times that how many the surfactant and how much the pH of medium should be. The optimum experimental conditions were determined. A new micellar enhanced spectrofluorimetric method for determination of lycopene was developed. The linear relationship between fluorescence intensity and concentration of lycopene is kept in the range of 9.7 pg/mL-85.9 ktg/mL. The detection limit is 1.1 pg/mL. The method has been applied to the determination of lycopene with satisfactory results.

关 键 词：胶束增敏荧光法 番茄红素 十六烷基三甲基溴化铵 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]