Dynamics of Interstitial Calcium in Rat Myocardial Ischemia Reperfusion Injury In Vivo  

作　　者：黄邵洪 宋江平 覃杰 荣健 吴钟凯 

机构地区：[1]Department of Cardiothoracic Surgery, the Third Affiliated Hospital Sun Yat-sen University, Guangzhou 510630, China [2]Department of Cardiovascular Surgery, State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital & Cardiovascular Inatiture, Beijing 10003 7, China [3]Department of Radiology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China [4]Department of Anesthesiology,the First Affiliated Hospital, Sun Yat-sen University, Guangzhou510089, China [5]Department of Cardiac Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou510089, China

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2014年第1期37-41,共5页华中科技大学学报（医学英德文版）

基　　金：supported by grants from the National Natural Science Foundation of China(No.81371714);the Natural Science Foundation of Guangdong Province(No.S2012010008151)

摘　　要：Intracellular calcium overload is a key factor for myocardial ischemia reperfusion injury（IR）. However, there was no report for interstitial calcium concentration dynamics. We investigated the interstitial calcium dynamics in rat myocardial IR model in vivo. A microdialysis system was involved, and the time delay of the system and recovery time was introduced and tested with a fluids switching method. Twelve SD rats were divided into IR or control group. Myocardial IR was induced by ligating（20 min） then releasing（60 min） the suture underlying left anterior descending branch. Mycrodialyisis probe was implanted into the left ventricular myocardium perfusion area for occlusion. Dialysate samples were collected every 10 min. Dialysate calcium concentration was detected with an atomic absorption spectrophotometer. Recovery time for the microdialysis system was 20 min, and recovery rate was 16%. Dialysate calcium concentration showed no changes during ischemia, descended immediately after reperfusion, reached the lowest level（67% of baseline value） 20 min after reperfusion, then escalated slowly. Recovery time was an important parameter for mycrodialysis technique, and it should not be neglected and needed to be tested. Our data suggest that interstitial calcium concentration in rats with myocardial IR in vivo kept steady in ischemia, descended rapidly at the initial reperfusion, then rebounded slowly. In conclusion, we introduced the concept of recovery time for microdialysis and provided a simple testing method.Intracellular calcium overload is a key factor for myocardial ischemia reperfusion injury（IR）. However, there was no report for interstitial calcium concentration dynamics. We investigated the interstitial calcium dynamics in rat myocardial IR model in vivo. A microdialysis system was involved, and the time delay of the system and recovery time was introduced and tested with a fluids switching method. Twelve SD rats were divided into IR or control group. Myocardial IR was induced by ligating（20 min） then releasing（60 min） the suture underlying left anterior descending branch. Mycrodialyisis probe was implanted into the left ventricular myocardium perfusion area for occlusion. Dialysate samples were collected every 10 min. Dialysate calcium concentration was detected with an atomic absorption spectrophotometer. Recovery time for the microdialysis system was 20 min, and recovery rate was 16%. Dialysate calcium concentration showed no changes during ischemia, descended immediately after reperfusion, reached the lowest level（67% of baseline value） 20 min after reperfusion, then escalated slowly. Recovery time was an important parameter for mycrodialysis technique, and it should not be neglected and needed to be tested. Our data suggest that interstitial calcium concentration in rats with myocardial IR in vivo kept steady in ischemia, descended rapidly at the initial reperfusion, then rebounded slowly. In conclusion, we introduced the concept of recovery time for microdialysis and provided a simple testing method.

关 键 词：MYOCARDIUM ISCHEMIA REPERFUSION CALCIUM MICRODIALYSIS 

分 类 号：R540.2[医药卫生—心血管疾病]