Nisin和柠檬酸对纯培养及鲜切黄瓜中单增李斯特菌的杀菌效果  

The disinfection effect of nisin and citric acid against Listeria monocytogenes in pure culture and on fresh-cut cucumbers

作　　者：陈晨[1] 胡文忠[1] 何煜波[1] 姜爱丽[1] 萨仁高娃[1] 

机构地区：[1]大连民族学院生命科学学院,生物化学工程国家民委-教育部重点实验室,辽宁省食源性病原微生物快速检测与控制工程技术研究中心,辽宁大连116600

出　　处：《食品工业科技》2014年第5期273-276,共4页Science and Technology of Food Industry

基　　金：“十二五”国家科技支撑计划项目(2012BAD38B05);国家自然科学基金资助项目(31172009);大连市科技计划项目(2012E13SF106);大连民族学院博士启动基金项目(0701-110014);中央高校基本科研业务费项目(DC2013010107)

摘　　要：本文研究了不同浓度的乳酸链球菌素(nisin)、柠檬酸及二者复配对纯培养和人工接种到鲜切黄瓜上的单增李斯特菌(LM)处理不同时间后的杀菌效果。结果显示nisin和柠檬酸对纯培养及鲜切黄瓜上的LM均具有一定的杀菌效果,其杀菌能力随着nisin和柠檬酸浓度的增加和处理时间的延长而增强。当nisin浓度为100μg/mL时,作用5min可使纯培养体系中的LM低于检测限,使鲜切黄瓜中的LM低于检测限需要15min,当柠檬酸浓度为0.5%时,分别作用10min和15min可以使纯培养和鲜切黄瓜中的LM低于检测限。nisin和柠檬酸复配的杀菌效果要优于二者单独使用,当二者复配浓度为50μg/mL+0.3%(m/v)时,处理20min可以使纯培养体系中的LM降低到检测限以下,而使鲜切黄瓜中的LM低于检测限则需15min。结果表明nisin和柠檬酸复配可以用于控制鲜切黄瓜中单增李斯特菌的污染。The disinfection effect of nisin and citric acid separately and in combination at different concentration for inactivation of Listeria monocytogenes （LM）in pure culture and on fresh -cut cucumbers was investigated.The results showed that both nisin and citric acid possessed considerable disinfection effect against LM in pure culture and on fresh-cut cucumbers, respectively, and the anti-listerial activity increased with the increasing concentration and treatment time.LM in pure culture was under the limit of detection within 5min by 100μg/mL nisin,while it need ;5min to reduce to that lever on fresh-cut cucumbers.LM was reduce to the lever that under the limit of detection by 0.5% citric acid within ;0min in pure culture and within 15min on fresh-cut cucumbers.The anti-listerial activity of the combination of nisin with citric acid was stronger than that of their alone.Combination of 50μg/mL nisin with 0.3% citric acid reduced LM to the lever that under the limit of detection in pure culture within 20min and on fresh- cut cucumbers within 15min.ln a conclusion,washing with nisin and citric acid might be applied as an effective preservative to control the growth of Listria monocytogene on fresh-cut cucumbers.

关 键 词：单增李斯特菌 乳酸链球菌素 柠檬酸 鲜切黄瓜 杀菌效果 

分 类 号：TS201.3[轻工技术与工程—食品科学]