山桃稠李果实花色苷对Nrf2/Keap1通路的影响  

Effect of Prunus Maackii fruit anthocyanins on Nrf2/Keap1 pathway

作　　者：刘荣[1] 姜元松[1] 辛越[1] 王蕾[1] 杨巍巍[1] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040

出　　处：《食品工业科技》2014年第6期116-118,124,共4页Science and Technology of Food Industry

基　　金：中央高校基本科研业务费专项资金(DL12CA11)

摘　　要：目的:探讨山桃稠李果实花色苷对Nrf2/Keap1通路的影响。方法:质量浓度为0.05、0.2、0.8mg/mL的山桃稠李果实花色苷分别作用于HepG2细胞24、48、72h,MTT法检测对HepG2细胞生长抑制率,检测各组细胞内谷胱甘肽(GSH)含量及谷胱甘肽硫转移酶(GSH-ST)、磷酯酰肌醇-3-激酶(PI3K)活性变化;实时荧光定量RT-PCR实验方法检测山桃稠李果实花色苷对HepG2细胞的Nrf2、Keap1、GSTP1的基因表达的影响。结果:随着花色苷质量浓度的增加,HepG2细胞的谷胱甘肽(GSH)含量下降,谷胱甘肽硫转移酶(GSH-ST)、磷酯酰肌醇-3-激酶(PI3K)活性显著降低;Nrf2、Keap1、GSTP1的基因表达下调。结论:山桃稠李果实花色苷能够通过对Nrf2/Keap1通路的调节来降低HepG2细胞的抗氧化能力。Objective：To study the effect of Prunus maackii fruit anthocyanins on Nrf2/Keapl pathway. Methods ： The HepG2 cells were treatet with concentration of 0.05,0.2,0.8mg/mL Prunus maackii fruit anthocyanins solution for 24,48,72h respectively. The growth inhibition rate of HepG2 cells and the content of glutathione （GSH）,activity of phosph oinositol-3-kinase（PI3K） of each group cells were determined by the method of MTT. The real-time fluorescent quantitative RT-PCR method was used to study the effect of Prunus maackii fruit anthocyanins on Nrf2,Keapl ,GSTP1 in HepG2 cells in genetic expression. Results：The results showed that as the content of anthocyanins increased,the content of GSH of HepG2 cells deceased,and the activity of glutathione s-transferases（GSH-ST） and PI3K decreased significantly. The Nrf2,Keapl ,GSTP1 gene expression level dropped. Conclusion ：the Prunus maackii fruit anthocyanins could reduce antioxidant capacity of HepG2 cells by adjusting the Nrf2/Keapl pathway.

关 键 词：山桃稠李 花色苷 HEPG2细胞 NRF2 KEAP1 

分 类 号：TS201.1[轻工技术与工程—食品科学]