大豆活性肽的安全性评价  

Safety assessment of soybean bioactive peptides

作　　者：高梦妮 江连洲[1] 朱秀清[2] 侯俊财[1] 张佳秀[1] 曹秋阁[1] 韩巍巍[1] 李晓静[1] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]国家大豆工程技术研究中心,哈尔滨150030

出　　处：《食品科技》2014年第3期266-270,共5页Food Science and Technology

基　　金："十二五"农村领域国家科技计划课题项目(2012BAD34B04)

摘　　要：旨在探讨风味蛋白酶酶解的大豆活性肽的急性毒性和遗传毒性。采用Ames试验、小鼠骨髓嗜多染红细胞微核试验、彗星试验、小鼠睾丸精母染色体畸变试验对大豆活性肽进行急性毒性分级和遗传毒性研究。试验结果表明,小鼠经口染毒最大耐受量(MTD)为20 g/kg,毒性分级为无毒;Ames试验鼠伤寒沙门菌突变型各菌株在加与不加S9的情况下,试验结果均呈阴性(P>0.05);小鼠骨髓细胞微核试验结果为阴性(P>0.05),各大豆活性肽剂量组动物未见细胞毒性作用;小鼠睾丸精母细胞畸形试验结果表明大豆肽剂量组与阴性对照组相比差异均不显著(P>0.05),且无量效关系。试验结果显示,大豆活性肽是一种安全无毒物质。The aim of the study was to investigate the acute and genetic toxicity of soybean bioactive peptides. The acute toxic test, Ames test, micronucleus test, comet assay, spermatogonial chromosomal aberrations test were used to evaluate the acute toxicity classification and genetic toxicity of soybean bioactive peptides. Results showed that the oral maximum tolerance dose of mice （MTD） was 20 g/kg, and the tested soybean bioactive peptides belong to non-toxic. The results of Ames test were negative in Salmonella typhimurium at any of the concentrations tested with or without an $9 activation system （P〉0.05）. Soybean bioactive peptides did not exhibit any cell toxicity in mouse bone marrow cells in a micronucleus assay with negative results of micronucleus test （P〉0.05）. There were not significant difference in the chromosomal abnormality rate and spermatogonial chromosomal aberrations test between the soybean bioactive peptides and the negative control.

关 键 词：大豆活性肽 急性毒性 AMES试验 微核试验 彗星试验 睾丸精母细胞染色体畸变试验 

分 类 号：TS201.21[轻工技术与工程—食品科学]