真菌毒素人工抗原制备方法研究进展  

Recent Progress in the Preparation of Artificial Antigen of Mycotoxins

作　　者：吴春生[1] 马良[1,2,3] 胡媛媛[1] 张宇昊[1,2,3] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]农业部农产品贮藏保鲜质量安全风险评估实验室(重庆),重庆400716 [3]重庆市特色食品工程技术研究中心,重庆400716

出　　处：《食品科学》2014年第5期239-245,共7页Food Science

基　　金：国家重点基础研究发展计划(973计划)项目(2013CB127803);国家自然科学基金项目(31301476);中央高校基本科研业务费专项资金项目(XDJK2013B035)

摘　　要：基于免疫原理的酶联免疫吸附技术和金标免疫层析技术等是目前真菌毒素主要的快速检测技术。人工完全抗原的制备和抗体的性质是免疫快速检测技术中最关键的因素。本文对我国粮油产品危害较大的重点真菌毒素,综述目前国内外其各种人工抗原的制备/合成技术,并分析存在的问题和解决方法,为小分子污染物人工完全抗原的定向制备以及获得高亲和性高效价抗体提供一定的理论基础。The main analytical techniques currently available for rapid detection of mycotoxins include enzyme-linked immunosorbent assay （ELISA） and gold-labeled immunochromatography. The synthesis of artificial antigen and the nature of antibodies are the most critical factors in rapid immunological detection of mycotoxins. This paper reviews the recent progress at home and abroad in the preparation/synthesis of artificial antigens of mycotoxins as hazardous contaminants of cereal and oil products. In addition, some existing problems and corresponding solutions are pointed out. This review will hopefully provide a theoretical basis for preparing artificial antigens of small molecule contaminants and develop the antibodies with high affinity and specificity.

关 键 词：真菌毒素 半抗原 人工抗原 制备 

分 类 号：TS201.6[轻工技术与工程—食品科学]