黄海黄杆菌YS-9412-130固定化细胞产酶技术  

Studies on enzyme production technology of immobilized cell from F. yellowsea YS 9412 130

作　　者：张云波[1] 王跃军 洪义国[2] 孙谧 刘惠[3] 王春波 刘晓萍[4] 

机构地区：[1]中国水产科学研究院黄海水产研究所海洋酶与酶工程开放实验室,青岛266071 [2]青岛海洋大学海洋生命学院,266003 [3]中国科学院生物工程中心,上海200233 [4]青岛大学医学院,266021

出　　处：《海洋水产研究》2000年第4期36-40,共5页Marine Fisheries Research

基　　金：国家"海洋 86 3"项目 !(86 3-819-0 6 -0 1)资助

摘　　要：3种不同的包埋材料固定化 YS- 94 12 - 130菌体细胞 ,进行半连续发酵。结果表明 ,用 2 .5%卡拉胶固定化细胞 ,产酶效率最高 ;添加明胶对产酶不利而添加豆饼粉与玉米粉后 ,酶活力有显著提高 ;卡拉胶固定化细胞发酵液中游离菌体浓度随发酵时间变化略有上升 ,总体水平较低。发酵液酶活随菌体浓度的增大而呈上升趋势 ;固定化细胞半连续发酵效率远高于游离细胞分批发酵的效率。The cells of F. Yellowsea YS 9412 130 immobilized with three different imbedding materials are fermented semi continuously. The result shows that the enzyme producing rate reaches the highest value when cells are immobilized with 2 5% carrageenan, adding soy bean cake meal and cornsteep meal can improve the enzyme activity greatly, while adding gelalin is bad to enzyme activity; the concentration of free cell in the fermented broth of carrageenan immobilized cells arises slightly with fermentation time going, however the level in general is low; the enzyme activity of fermented broth shows a rising trend with the increase of the cells concentration; the efficiency of semi continuous fermentation of immobilized cells is much higher than that of batch fermentation with free cells.

关 键 词：卡拉胶 固定化细胞 黄海黄杆菌 产酶菌 发酵工艺 

分 类 号：TQ925.2[轻工技术与工程—发酵工程] Q814.2[生物学—生物工程]