微波辅助提取瓜蒌皮多糖及抗氧化活性  

Microwave-assisted extraction of polysaccharides in Trichosanthes kirilowii peel and its antioxidant activity

作　　者：杨柳[1] 张敏[1] 李军红[1] 太史超凡 叶明[1] 卢向中 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]安徽卢氏生态农业科技有限责任公司,安徽池州247100

出　　处：《合肥工业大学学报（自然科学版）》2014年第3期359-363,共5页Journal of Hefei University of Technology：Natural Science

基　　金：安徽省科技攻关重点资助项目(12010302071)

摘　　要：文章以瓜蒌皮为原料,采用响应面分析法对微波辅助提取瓜蒌皮多糖提取条件进行优化,并从还原力、·OH和NO2-·清除率等方面对瓜蒌皮多糖的抗氧化活性进行了研究。结果表明,当料液比为1∶40、微波提取时间为15min、微波提取功率为450W时,瓜蒌皮多糖得率最高,为2.52%。当瓜蒌皮多糖溶液质量浓度为1.2g/L时,其还原力为0.54,对·OH的清除率为72.8%,对NO2-·的清除率为35.6%。因此,瓜蒌皮多糖是一种天然的抗氧化剂。Trichosanthes kirilow ii peel were used to extract polysaccharides by microwave-assisted ex-traction .The optimum microwave-assisted extraction conditions were obtained through response sur-face analysis .The antioxidant activity of Trichosanthes kirilowii peel polysaccharides was evaluated by total reducing power ,hydroxyl radical scavenging assay and NO2 - scavenging assay .The results showed that the optimal extraction conditions were as followed ：the material-to-liquid ratio was 1∶40 ,the extraction time was 15 min ,and the microwave power was 450 W .Under these extraction conditions , the rate of polysaccharides yield were 2.52% .When its solution concentration was 1.2 g/L ,the re-ducing power ,the hydroxyl radical scavenging rate and the NO2 - scavenging rate of the polysaccha-rides were 0.54（OD） ,72.8% and 35.6% respectively .Trichosanthes kirilowii peel polysaccharide was a kind of natural antioxidant .

关 键 词：瓜蒌皮多糖 响应面分析 微波辅助提取 抗氧化活性 

分 类 号：TS201.23[轻工技术与工程—食品科学]