茶树EST序列中微卫星标记的特征分析及应用  

Characteristic Analysis and Application of Microsatellites from EST Sequence of Camellia sinensis

作　　者：严伟[1] 王婧[1] 丁华[1] 周有祥[1] 赵明明[1] 胡定金[1] 

机构地区：[1]湖北省农业科学院农业质量标准与检测技术研究所,武汉430064

出　　处：《湖北农业科学》2013年第24期6178-6181,共4页Hubei Agricultural Sciences

基　　金：公益性行业(农业)专项(201203046);湖北省农业科技创新中心项目(2013-620-000-001);湖北省农业科学院青年基金项目(2012NKYJJ18)

摘　　要：从NCBI数据库中获得47 913条茶树(Camellia sinensis)EST序列,通过Cd-hit-est除去冗余序列后得到26 185条非冗余序列,利用Misa软件分析后共发现8 244个微卫星标记分布于6 283条EST序列中,平均相隔1.52 kb出现1个SSR,EST-SSR序列出现频率为23.99%。其中,单、二和三核苷酸重复为主导类型,占EST-SSR总数的97.83%。A/T、AG/CT和AAG/CTT作为单、二和三核苷酸的优势重复基元,分别占所属类型的92.99%、85.11%和26.99%。利用Primer 5.0软件,设计了76对引物进行多态性检测,发现其中59对引物可以扩增出明显的条带,34对引物具有多态性,多态性比率高达57.63%,表明茶树EST-SSR中的多态性位点比较丰富,比较适合进行大规模的EST-SSR多态性标记的筛选。A total of 26 185 non-redundant EST sequences were obtained from 47 913 sequences by redundant analysis from NCBI.8 244 EST-SSR presenting in 6 283 EST out of the non-redundant sequences with one EST-SSR in every 1.52 kb of EST sequence on average.The major types were mononucleotide,dinucleotide and trinucleotide repeats accounting for 97.83％ of all the SSR.A/T,AG/CT and AAG/CTT were the dominating motifs accounting for 92.99％,85.11％ and 26.99％ in mononucleotide,dinucleotide and trinucleotide repeats,respectively.76 pairs of SSR primers were designed with Primer 5.0.59 primer pairs can be amplified with clear band and 34 primer pairs are polymorphic.The proportion of primers producing polymorphic fragments was 57.63％.The results showed that the microsatellites screened were useful for the further study.

关 键 词：茶树(Camellia sinensis) EST-SSR 分布特征 

分 类 号：S571.1[农业科学—茶叶生产加工]