重组大肠杆菌产青霉素酰化酶的发酵动力学研究  

Fermentation kinetics of penicillin acylase produced by recombinant Escherichia coli

作　　者：韦晓菊[1] 黎继烈[1] 朱晓媛[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院经济森林培育与保护教育重点实验室,湖南长沙410004

出　　处：《中国酿造》2014年第3期32-35,共4页China Brewing

基　　金：国家林业局“948”项目(NO.2011-4-17)

摘　　要：对产青霉素酰化酶重组大肠杆菌M3进行分批发酵动力学研究。根据Logistic和Luedeking-Piret方程,拟合出重组大肠杆菌M3分批发酵过程中菌体生长、青霉素酰化酶合成、基质消耗的动力学模型及模型参数。结果表明,重组大肠杆菌M3产青霉素酰化酶的发酵动态过程能很好的被该模型所表达。The fermentation kinetics for penicillin acylase production by the recombinant Escherichia coil M3 was studied in this paper. Based on the Logistic and Luedeking-Piret equation, the kinetic models of recombinant E. coli M3 about microorganism growth, penicillin acylase formation, sub- strate eonsumption and model parameter were proposed. The result showed that the fermentation process of penicillin acylase produced by recombi- nant E. coli M3 could be well expressed by the model.

关 键 词：青霉素酰化酶 分批发酵 发酵动力学模型 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]