常压室温等离子体诱变筛选高乳糖酶活力酵母的研究  

Research on Mutation of High Lactase Activity Kluyveromyces lactis by ARTP

作　　者：邱雯雯[1] 任雅琳[1] 陈存社[1] 杨菊芳[1] 吴昊[1] 董丽艳[1] 

机构地区：[1]北京工商大学食品添加剂与配料北京高校工程研究中心,北京100048

出　　处：《中国食品学报》2014年第2期132-137,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：北京市教委科技发展计划项目(KZ20110011013);学科与研究生教育-重点学科-应用化学资助项目(PXM2012_014213_000038)

摘　　要：探讨常压室温等离子体(ARTP)诱变筛选高乳糖酶活力乳酸克鲁维酵母的条件。以ARTP诱变育种方法为诱变手段,对乳酸克鲁维酵母进行不同时长(10 s依次增加到300 s)的诱变处理,并结合高通量筛选方法快速筛选出33株乳糖酶活力提高50%以上的突变菌株。通过复筛最终得到4株高乳糖酶活力的突变菌株,采用三角瓶摇床培养,结果表明:筛选得到的突变菌株的乳糖酶活力均大于原始菌株,其中最大乳糖酶活力提高到0.257 U/mL,是原始菌株(0.090 U/mL)的2.8倍;4株突变菌株生长速度比原始菌株显著提高。ARTP诱变育种技术结合高通量筛选方法是1种快速、有效的新型微生物诱变育种方法。The experiment explored conditions for mutation and screening of high lactase activity Kluyveromyces lactis by atmospheric and room temperature plasmas（ARTP）. With ARTP mutagenic method and high throughput screening method, Kluyveromyces lactis was mutagenized for different time（stages range from 10s up to 300s）, and 33 strains of mutant passed the screening with condition of more than 50% increase in lactase activity as screening condition. Four mutants obtained after rescreening was put into culture for their high lactase activity. The result shows that： all mutant obtained from the screening has higher lactase activity than original strain, and the maximize lactase activity is 0.257 U/ mL, 2.8 times of original strain while grow speed of the four strains are more faster. The research reveals mutation by ARTP conditions combined with high throughput screening method is a new high speed microbial mutagenize method.

关 键 词：常温室压等离子体 诱变 乳糖酶 乳酸克鲁维酵母 

分 类 号：TS201.3[轻工技术与工程—食品科学]