猪肉中多种兽药残留液质联用检测技术的研究  

Determination of Residues of Veterinary Drug in Pork by LC-MS/MS

作　　者：张骏[1,2] 王硕[1] 张燕[1] 何佳[3] 林安清[3] 

机构地区：[1]食品营养与安全省部共建教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457 [2]天津市质量技术监督局,天津300100 [3]天津出入境检验检疫局,天津300461

出　　处：《食品研究与开发》2014年第6期70-74,136,共6页Food Research and Development

基　　金：863计划(No 2011AA100806);国家质检总局重点科研项目(No 2007IK131)

摘　　要：建立了可同时检测猪肉中氯丙嗪，丙酰丙嗪，氮哌酮，氟哌啶醇，氮哌醇，咔唑心安多种兽药残留的液相色谱-串联质谱（LC-MS /MS）方法，在已有的研究基础上对样品的前处理方法进行了优化和改进，检出限均为0.5μg/kg，平均加标回收率在77.2%～100.3%之间，相对标准偏差（RSD）在4.7％～10.1％之间。该方法简单快速，回收率高，重现性好，方法回收率和精密度满足残留分析规定指标。A liquid chromatography-electrospray ionization tandem mass spectrometry （LC-MS/MS） method for the determination of some residues of veterinary drug （chlorpromazine, azaperone, propionylpromazine, haloperidol, azaperol and carazolol） in pork was developed. The research optimized and improved the sample pretreatment on the basis of existing study. All of the limits of detection （LODs） of these veterinary drug were 0.5μg/kg. The average recoveries of target drugs ranged from 77.2%-100.3%with relative standard deviations （RSDs） of 4.7%-10.1%. The developed method is the suitable method for the rapid quantitative and reliable determination of veterinary drug residues. The results demonstrated that the sensitivity , accuracy and precision of this method meet the requirements of residue analysis. The method is applicable to detect these residues of veterinary drug in pork.

关 键 词：猪肉 兽药 质谱 检测 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]