酪蛋白磷酸肽副产物中ACEI肽的富集工艺研究  

Enrichment process of angiotensin I-converting enzyme inhibitory peptides from casein peptide phosphate by-products

作　　者：赵力超[1] 宁德山 李双祁 杜洁[1] 王燕[1] 曹庸[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642 [2]无限极(中国)有限公司,广东广州510623 [3]广州绿萃生物科技有限公司,广东广州510663

出　　处：《食品工业科技》2014年第11期238-242,共5页Science and Technology of Food Industry

基　　金：广东省科技计划项目(2012B020312004)

摘　　要：以工厂生产酪蛋白磷酸肽后的副产物(CPPBP)为原料,在研究其分离特性的基础上,初步去杂,并借助马尿酸法和HPLC法定位活性最高多肽的位置。接着,以高活力ACEI肽得率为指标,设计三因素三水平的正交实验优化富集工艺,最终研发一种二段量化富集ACEI肽的新工艺。该工艺第一段以CPPBP饱和多肽液为原料,设定沉淀条件为pH2、-16℃、沉淀1h,得到粗富集物(P粗)。第二段再以P粗饱和液为原料,在pH4、乙醇浓度70%、-20℃条件下,沉淀1h。最后产物得率为19.11%,经活性测定,ACE抑制率为46.29%。In this paper,a novel two-stage enrichment process was developed for mass-producing ACE-inhibitory peptides by using casein peptide phosphate production by-products （CPPBP）.After pre-concentrating active peptides on the basis of analyzing separation properties of CPPBP,the high efficient components were located by hippuric acid method and HPLC method.And then a three factor and three levels orthogonal experiment,taking yield of high efficient components as index,was designed to optimize the process conditions of concentrating ACE -inhibitory peptides.ln first stage, saturated CPPBP solution was used as material and precipitation conditions were set as pH2 and-16℃ for 1h.The pre-concentration was named as Pcrude.ln second stage,the final product was precipitated from saturated Pcrude under pH4, Ethanol concentration 70% and -20℃; for 1h.The yield of enrichment process was 19.11% with an inhibition of 46.29% to ACE.

关 键 词：ACEI 多肽 富集 工艺设计 废弃物再利用 

分 类 号：TS201.2[轻工技术与工程—食品科学]