超声波辅助法协同酶解提取平菇水溶性多糖的工艺研究  

Study on Enzymolysis Extraction Process of Water Soluble Polysaccharide from Pleurotus ostreatus by Ultrasonic-assisted Method

作　　者：黄雅君[1] 毕红梅[2] 郑文凤[2] 周艳丽[1] 吴丹丹[1] 兰小兵[1] 梁英[2] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]黑龙江八一农垦大学理学院

出　　处：《黑龙江八一农垦大学学报》2014年第3期35-39,共5页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省垦区科研项目(HNK13KF-01-04);黑龙江省教育厅大学生创新性实验训练计划项目

摘　　要：采用超声波辅助法协同酶解提取平菇水溶性多糖。在单因素试验的基础上,采用正交试验分别研究液固比、酶解pH值、酶解温度、超声时间对平菇多糖浸提率的影响,最佳工艺条件。结果表明:提取平菇多糖的最佳工艺是:液固比30∶1,酶解pH值6,酶解温度60℃,超声时间40 min。在此条件下,超声波辅助法协同酶解提取平菇多糖的浸提率为20.92%。Ultrasonic-assisted enzymolysis was used to extract water soluble polysaccharide from Pleurotus ostreatus. On the basis of single factor experiments, the optimum process conditions of liquid-solid ratio, enzymolysis pH, enzymolysis temperature, ultrasonic time were studied by orthogonal tests. The optimum extraction conditions of polysaccharide from Pleurotus ostreatus was as follows：the liquid-solid ratio 30∶1,enzymolysis pH 6,enzymolysis temperature 60 ℃,ultrasonic time 40 min. Under the above conditions,the productivity of enzyme extraction of water soluble polysaccharide from Pleurotus ostreatus was 20.92%by ultrasonic-assisted method.

关 键 词：平菇 水溶性多糖 超声波 酶解 

分 类 号：TS202.3[轻工技术与工程—食品科学]