微乳液和微乳液凝胶中脂肪酶催化的酯合成反应  

Esterification Reaction Catalyzed by Lipase in W/O Microemulsions and Microemulsion-Based Organogels

作　　者：周国伟[1] 李干佐[1] 李越中[1] 徐健[1] 王仁会[1] 李传光[1] 

机构地区：[1]山东大学

出　　处：《化学学报》2001年第3期344-349,共6页Acta Chimica Sinica

基　　金：国家自然科学基金

摘　　要：在AOT/异辛烷/水形成的油包水微乳液中，研究了Candida lipolytical(CL)脂肪酶催化庚酸和庚醇的酯化反应，动力学研究表明反应符合乒乓(Ping-Pong)Bi Bi机制，两底物酸和醇均有抑制效应，并测定了反应的表观动力学常数.将CL脂肪酶固定于含明胶的微乳液凝胶(MBGs)中，可制得固定化脂肪酶，含酶的MBGs在非极性有机溶剂中可作为一种新的固相催化剂.并研究了MBGs在异辛烷中催化合成酯反应的性能，所制得的MBGs重复利用性和贮存稳定性都非常好.Esterification reactions of heptanoic acid with heptanol, catalyzed by Candida lypolytical (CL) lipase, was studied in water - in - oil microemulsions formed by water/bis - (2 - ethylhexyl) sulfosuccinate sodium(AOT)/isooctane. Kinetic studies showed that the reaction follows a Ping - Pong Pi Bi mechanism with inhibition by both substrates. The values of all apparent kinetic parameters were determined. Lipase has also been immobilized in gelatin - containing AOT microemulsion - based organogels(MBGs) without any loss of catalytic activity. These lipase - containing MBGs prove to be novel solid - phase catalysts for use in apolar organic solvents and have good storage stability. These lipase - containing MBGs retained its higher activity after many runs esterification reactions.

关 键 词：油包水微乳液 酶催化 固定化本科 微乳液凝胶 脂肪酶 庚酸 庚醇 酯化反应动力学 

分 类 号：O621.251[理学—有机化学] O621.256.4[理学—化学]