河北环渤海地区补血草不同部位黄酮类化合物的研究  

Flavonoids Analysis in Different Parts of the Limonium spp. in the Coastal Region of Heibei

作　　者：张玲[1] 李岩[1] 田文睿 王向红[1] 

机构地区：[1]河北农业大学食品科技学院河北省农产品加工工程技术研究中心,河北保定071001

出　　处：《中国食品学报》2014年第4期57-63,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：河北省科技支撑计划项目(12277117D)

摘　　要：目的：以补血草为原料，采用超声法提取醇溶性物质，对提取物中的黄酮类化合物进行研究。方法：石油醚脱脂后，以甲醇为溶剂超声提取醇溶性物质，提取物减压浓缩除去有机溶剂，经真空冷冻干燥后得到粗提物。取粗提物，加80％甲醇和2mL4．0mol／L HCl，70℃水解4h，减压浓缩除去水解液，得到黄酮苷元。用高效液相色谱法测定补血草根、茎、叶、花中黄酮类化合物槲皮素、山奈酚、异鼠李素、儿茶素及没食子酸的含量。结果：补血草根、茎、叶和花各个部位没食子酸和儿茶素含量较高，为6．33～1．56mg／100g；补血草叶中山奈酚含量为0．16mg/100g，其余部位未检测出；补血草根中未检测出槲皮素，其余部位含量为0．3～5．81mg／100g。结论：补血草各部位儿茶素和没食子酸含量都明显高于槲皮素和山奈酚。Objective： In this papaer, useing the Limonium spp. as raw materials, alcohol-soluble material was ex- tracted by ultrasonic extraction. Flavonoids extract was analysised. Methods： Extract was concentrated under reduced pres- sure to remove the organic solvent, then by vacuum freeze-drying moisture was removed. Dried residue （50 mg） was weighed into a 10-mL test tube then dissolved in 8mL 80% MeOH and 2 mL g.0 mol/L HC1. Hydrolysis was carried out in a water bath at 70℃ for g h. Flavonoid compounds： quercetin, kaempferol, isorhamnetin, catechin and gallic acid content in roots, stems, leaves, flowers of Limonium spp. was determined by high performance liquid chromatography. Results： Isorhamnetin was not detected in roots, stems, leaves, flowers of Limonium spp. The cntent of gallic acid and catechin in all parts were high： 6.33-1.56 mg/（100 g）. Kaempfer--1 content in leaves of the Limonium spp. was 0.16 mg/ （100 g）, but remaining parts were not detected. Quercetin was not detected in root. Contents of other parts were 0.3- 5.81 mg/（100 g）. Conclusion： The catechins and gallic acid content in various parts of the Limonium spp were signifi- cantly higher than that of quercetin and kaempferol content and various parts of isorhamnetin were not detected.

关 键 词：补血草 高效液相色谱 槲皮素 山奈酚 异鼠李素 儿茶素 没食子酸 

分 类 号：TS201.2[轻工技术与工程—食品科学]