菜籽分离蛋白分子质量分布及酶解条件的研究  

Molecular Weight Distribution of Rapeseed Protein Isolate and Optimization of Enzymatic Hydrolysis Process

作　　者：高瑀珑[1] 郑锐[1] 王玉梅[1] 王立峰[1] 鞠兴荣[1] 袁建[1] 

机构地区：[1]南京财经大学食品科学与工程学院江苏省粮油品质控制及深加工技术重点实验室,南京210023

出　　处：《中国粮油学报》2014年第6期38-45,共8页Journal of the Chinese Cereals and Oils Association

基　　金：国家农业科技成果转化资金(2011GB2C100012;2012GB24490611);国家科技支撑计划(2012 BAD37B08);南京财经大学预研究项目(Y2012014)

摘　　要：以脱脂"双低"油菜籽为原料,利用碱溶解酸沉淀法提取菜籽分离蛋白;用SDS-PAGE凝胶电泳研究菜籽蛋白的分子质量的组成;以水解度和氮回收率为考察指标,用响应面分析法拟合了Alcalase 2.4L酶解菜籽蛋白成菜籽肽的二次多项数学模型,优化了酶解菜籽分离蛋白的工艺参数。SDS-PAGE凝胶电泳研究表明利用碱溶解酸沉淀提取的菜籽分离蛋白主要是2S清蛋白。响应面分析法研究的试验结果表明,酶的使用量、pH、酶解温度、酶的使用量与pH的交互作用对Alcalase 2.4L酶解菜籽蛋白的水解度和氮回收率的影响均显著(P<0.05)。通过求解菜籽肽的二次多项数学模型的逆矩阵方程,可得Alcalase 2.4L水解菜籽分离蛋白的最佳条件为:酶的使用量0.05 Au/g,pH 9.0,酶解温度54℃;在此酶解条件下,菜籽分离蛋白浓度为5%时,水解5 h,所得的水解度和氮回收率分别为35.12%及52.96%。Defatted meal of double -zero rapeseed has been adopted as material in the paper. The methods of alkaline extraction and acid precipitation were employed to extract rapeseed protein isolate; SDS -PAGE gel eleetrophoresis has been adopted to explore the molecular weight distribution. On the basis of single -factor experiments, response surface methodology （RSM） was utilized to built second - order polynomial equations and to optimize condi- tions of enzymatic hydrolysis process with Alcalase 2.4L; the selected response values were degree of hydrolysis （DH） and nitrogen recovery （NR）. Analyses by electrophoresis showed that this protein isolate was mainly composed of more 2S albumins than 12S globulins. The experimental results showed that adding enzyme amount, pH, tempera- ture and the interaction of enzyme amount and pH would have a significant effect on DH and NR （ P 〈 0.05 ）. Maxi- mum yield was obtained according to the inverse matrix equation of the second - order polynomial model when enzyme amount added; pH and temperature were 0. 05 Au/g, pH 9. 0, 54 ℃, respectively. The conditions resulted in 35.12% of DH, 52.96% of NR after 5 h hydrolysis with a rapeseed protein isolate substrate of 5%.

关 键 词：菜籽分离蛋白 SDS—PAGE电泳 酶解 水解度 氮回收率 响应面法 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程]