红缘拟层孔菌发酵物体外抗氧化活性研究  

Study of antioxidant activities of the fermentation products from Fomitopsis pinicola

作　　者：圣志存 郝利民[1,2] 陶如玉 余坚勇[2] 刘晋[2] 鲁吉珂[2,3] 贾士儒[1] 

机构地区：[1]天津科技大学,工业发酵微生物重点实验室,天津300457 [2]总后勤部军需装备研究所,北京100010 [3]郑州大学生命科学学院,河南郑州450001

出　　处：《食品工业科技》2014年第13期129-133,共5页Science and Technology of Food Industry

摘　　要：分别采用DPPH自由基、羟自由基清除能力和氧化损伤酵母保护能力实验,对红缘拟层孔菌发酵物的体外抗氧化活性进行研究。结果表明:红缘拟层孔菌发酵浓缩物和醇沉物具有显著清除自由基和保护细胞氧化损伤的作用,二者抗氧化活性与天然抗氧化剂BHT相近,并呈线性剂量依赖性。一定的质量浓度范围内,浓缩物和醇沉物对DPPH自由基的EC50分别为7.69和7.72mg/mL,羟基EC50值分别为4.42和4.44mg/mL,紫外氧化损伤酵母细胞EC50值分别为30.40和34.87mg/mL,H2O2氧化损伤酵母细胞EC50值分别为27.12和30.61mg/mL,说明红缘拟层孔菌发酵物具有显著的抗氧化活性。The antioxidant activities in vitro of fermentation products from Fomitopsis pinicola were investigated byscavenging DPPH, hydroxyl radical experiments and a protection test model of oxidative damage to yeast cells.Theresults indicated that both Fomitopsis pinicola fermentation concentrates and ethanol sediments had significanteffects on scavenging radicals and oxidative damages,even could compare with the activity of nature antioxidantBHT.Moreover,their scavenging abilities were positively correlated with the dose.The effective concentrations ofFPC and FPES that scavenged 50% （ECs0） DPPH were 7.69 and 7.72mg/mL, respectively,while that value were4.42, and 4.44mg/mL on hydroxyl,30.40mg/mL and 34.87mg/mL on UV oxidative damage,27.12 and 30.61mg/mLon H2O2 oxidative damage, respectively. So it can be concluded that the fermentation products of Fomitopsispinicola showed significant antioxidant activity.

关 键 词：红缘拟层孔菌 浓缩物 醇沉物 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]