利用非培养技术初步研究古井贡酒窖泥细菌群落结构  

Preliminary analysis on bacterial communities in GuJingGong pit mud through culture-free approach

作　　者：张会敏 李天婵 孙美青 周庆伍 李安军 何宏魁 张治洲 

机构地区：[1]哈尔滨工业大学(威海)海洋科学与技术学院,山东威海264209 [2]安徽古井贡酒股份有限公司,安徽亳州236826

出　　处：《食品工业科技》2014年第13期200-206,228,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金资助项目(31071170);GREDB10资金;HIT(hitwh200904);985海洋科学资金;HIT-NSRIF(2011101);威海科技发展项目(2011DXGJ13;2012DXGJ02)

摘　　要：采用构建16S rDNA克隆文库的非培养方法,对古井贡酒窖泥细菌的群落结构及其多样性进行研究。结果表明:文库含有57个分类操作单元(OTU),793个克隆中未知菌类占60.78%,其中5个克隆得不到任何比对信息,占0.63%。已知菌类分别属于Firmicutes(31.16%)、Proteobacteria(3.55%)、Bacteroidetes(3.79%)、Synergistetes(0.63%)、Actinobacteria的Atopobium(0.13%)。已知菌类也多半为非培养细菌,其中Firmicutes占多数,但是其中也有一半的细菌相似度偏低,需要进一步验证。16S rDNA clone library technique was conducted to analyze the bacterial community diversity in pit mudof GuJingGong liquor.All clone strains were classified to 56 OTUs.Of all the 793 clones,60.78% were unknownspecies,and 5 sequences（0.63% ）could not be found any information in Genebank.The known bacteria belongedto Firmicutes（ 31. 16% ）, Proteobacteria（ 3.55 % ）, Bacteroidetes （3.79%）, Synergistetes （0.63 % ） and Actinobacteria（0.13%）.Most of the known bacteria were also found as uncultured, of which, Firmicutes was the majority. Half ofFirmicutes bacteria require further verification since they had a relatively low similarity in BLAST analysis.

关 键 词：古井贡酒 窖泥 细菌群落 TA克隆 

分 类 号：TS201.3[轻工技术与工程—食品科学]