乳酸菌发酵剂浓缩培养的研究  

Study on the culture with concentration of lactic acid bacteria starter

作　　者：吕兵[1] 张国农[1] 

机构地区：[1]无锡轻工大学食品学院,江苏无锡214036

出　　处：《中国乳品工业》2001年第3期7-9,共3页China Dairy Industry

摘　　要：为制备高效浓缩乳酸菌发酵剂，通过综合运用复合生长培养基、缓冲盐法及化学中和法，对乳酸菌的浓缩培养进行了研究。试验结果表明，以 12％ NFM＋ 0.3％酵母膏＋ 0.1％ Tween 80作为复合生长培养基，加 0.5％ K2HPO4作为缓冲盐，接种量为 2％ (嗜热链球菌∶保加利亚乳杆菌 =1∶ 1)，培养温度 42℃，培养过程用 30％ Na2CO3溶液作中和剂，将 pH值控制在 6.3，培养 6～ 7 h后，可使乳酸菌的活菌数达到 5.89× 109 mL－ 1。与普通的液体发酵剂相比，获得了显著的浓缩效果。In order to prepare the high effective lactic acid bacteria starter concentrates,the culture with concentration of lactic acid bacteria was studied through using a compound medium,buffer salt and neutralizer in this paper.The experiment result indicate:The composition of the compound medium is 12％ NFM,0.3％ yeast extract,and 0.1％ Tween 80 with addition of a buffer salt of 0.5％ K2HPO4.Inoculum size is 2％ (S.t∶ L .b=1∶ 1).The incubation temperature is 42℃ .30％ Na2CO3 was used as a neutralizer to control pH at 6.3 during incubation.After growth for 6～ 7 h,the number of viable cells is 5.89× 109 mL－ 1 .A notable effect of concentration was gained on comparing with simple liquid starter.

关 键 词：乳酸菌发酵剂 浓缩培养 缓冲盐 中和剂 制备 

分 类 号：TS201.3[轻工技术与工程—食品科学] TS202.3[轻工技术与工程—食品科学与工程]