豆天蛾幼虫水解试验研究  

A Study on the Enzymatic Hydrolysis of Herse Bilineata Tsingtauica

作　　者：吴胜军[1] 江龙法[1] 陈绍军[2] 

机构地区：[1]淮海工学院食品工程系,江苏连云港222005 [2]福建农业大学食品科学系,福建福州350002

出　　处：《淮海工学院学报（自然科学版）》2002年第1期52-55,共4页Journal of Huaihai Institute of Technology:Natural Sciences Edition

基　　金：福建省科委基金资助项目 ( 1995 12 )

摘　　要：以豆天蛾幼虫为原料 ,用 AS1 .3 98中性蛋白酶、木瓜蛋白酶和复合风味蛋白酶为水解酶 ,分别研究其水解特性 ,最后确定复合风味蛋白酶为最优水解酶 ,其最优水解方案 :温度为 5 0℃、p H=6 .5、时间 1 0 h、加酶量为 40 LAPU/ g。按此方案进行水解 ,重复 3次 ,得到平均水解率为 3 2 .5 4 % ,蛋白质利用率为 82 .3 6 % 。In this paper, we used AS1.398 Neutrase, Papain and Flavourzyme to study the enzy\|matic hydrolysis of Herse bilineata tsingtauica . We confirmed that Flavourzyme was the best one. The optimum hydrolysis condition was as follows: hydrolysis temperature 50 ℃, pH=6.5, time 10 hours, the oil elimated Herse bilineata tsingtauica 5% and Flavourzyme weight 40 LAPU/g dry matter. After the hydrolysis process is repeated for three times, the best result can be achieved with the percentage of hydrolysis being 32.54% and the obtained protein being 82.36%.

关 键 词：豆天蛾 AS1.398中性蛋白酶 木瓜蛋白酶 复合风味蛋白酶 水解率 幼虫 蛋白水解性能 

分 类 号：Q969.436.2[生物学—昆虫学] TQ936.1[化学工程]