哈密瓜(Cucumis melo L.)致腐真菌的鉴定  

Identification of Spoilage Fungi of Cantaloupe (Cucumis melo L.)

作　　者：乔言平 吴朝霞[2] 丁真真[1,3] 王宇滨 马越[1,5] 赵晓燕 张超[1] QIAO Yan-ping;WU Zhao-xia;DING Zhen-zhen;WANG Yu-bin;MA Yue;ZHAO Xiao-yan;ZHANG Chao(Beijing Academy of Agriculture and Forestry Sciences,Beijing Vegetable Research Center,Beijing 100097,China;Shenyang Agricuhure University,Shenyang 110866,China;Kashi University,Kashi 844006,China;Beijing Key Laboratory of Fruits and Vegetable Storage and Processing,Beijing 100097,China;Key Laboratory of Vegetable Postharvest Processing of Ministry of Agriculture,Beijing 100097,China)

机构地区：[1]北京市农林科学院蔬菜研究中心,北京100097 [2]沈阳农业大学,辽宁沈阳110866 [3]喀什大学,新疆喀什844006 [4]果蔬农产品保鲜与加工北京市重点实验室,北京100097 [5]农业部蔬菜产后处理重点实验室,北京100097

出　　处：《食品工业科技》2018年第23期123-127,133,共6页Science and Technology of Food Industry

基　　金：科技部重点研发计划(2016YFD0400302-5);现代农业产业技术体系资助(CARS-23&25);北京市自然科学基金资助项目(6172013);果蔬农产品保鲜与加工北京市重点实验室资助项目(Z141105004414037)

摘　　要：研究从哈密瓜表面分离出引起其腐败的菌株A和菌株B,并采用显微镜形态学观察、表型特征和分子生物学三种方法对其进行鉴定。结果发现菌株A可利用碳源包括杏苷、糊精、D-半乳糖、D-葡萄糖酸、麦芽糖、肝糖、L-鼠李糖、L-山梨糖、L-乳酸等72种碳源,其ITS rDNA序列与尖孢镰刀菌甜瓜转化型(Fusarium oxysporum f.sp.melonis)的同源性为99%,分支长度为0.00。菌株B可利用碳源包括L-阿拉伯糖、D-果糖、丙三醇、蔗糖和木糖醇等54种碳源,其ITS rDNA序列与链格孢菌(Alternaria alternata)的同源性为100%,分支长度为0.00。因此,推断菌株A为尖孢镰刀菌甜瓜转化型,菌株B为链格孢菌。Strain A and B were isolated from the surface of the cantaloupe( Cucumis melo L.),which was further identified by the microscopic morphological observation,phenotypic characteristics and molecular methods. Strain A used the 72 kinds of carbon sources,including dextrin,D-galactose,D-gluconic acid,maltose,glycogen,L-rhamnose,L-sorbose,L-lactic acid and etc..ITS rDNA sequence of strain A showed 99% homology with Fusarium oxysporum f. sp. melonis with the branch length of0.00. Strain B used 54 kinds of carbon sources,including L-arabinose,D-fructose,glycerol,sucrose,xylitol and etc..ITS rDNA sequence strain B showed 100% homology with Alternaria alternata with the branch length of 0.00. Therefore,strain A and B was supposed to be Fusarium oxysporum f.sp.melonis and Alternaria alternata,respectively.

关 键 词：哈密瓜 致腐菌 ITS(Internal transcribed spacer)测序 BIOLOG PMs表型芯片系统 

分 类 号：TS201.1[轻工技术与工程—食品科学]