3种豆芽中异黄酮、多酚的体外抗氧化活性及其对果蝇SOD、GSH-Px活力的影响  

In vitro Antioxidant Capacities of Isoflavones and Polyphenols in Three Bean Sprouts and Their Effects on Activities of SOD and GSH-Px in Drosophila melanogaster

作　　者：王飞霞 杨晓华[2] 张华峰 朱春燕 李璐 王凤忠[3] Wang Feixia;Yang Xiaohua;Zhang Huafeng;Zhu Chunyan;Li Lu;Wang Fengzhong(College of Food Engineering and Nutritional Science,Shaanxi Normal University,International Joint Research Center of Shaanxi Province for Food and Health Sciences,Xi'an 710119;Health Science Center,Xi'an Jiaotong University,Xi'an 710061;Institute of Ago-products Processing Science and Technology,Chinese Academy of Agricultural Sciences Key Laboratory of Ministry of Agriculture for Agro-products Processing,Beijing 100193)

机构地区：[1]陕西师范大学食品工程与营养科学学院,中俄食品与健康科学国际联合研究中心,西安710119 [2]西安交通大学医学部,西安710061 [3]中国农业科学院农产品加工研究所,农业部农产品加工重点实验室,北京100193

出　　处：《中国食品学报》2018年第11期57-64,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：中央高校基本科研业务费专项资金项目(GK201703076);陕西省科学技术研究项目(2016SF-350,2018ZDXM-SF-006);农业部农产品加工重点实验室开放课题(2015010).

摘　　要：采用高效液相色谱(HPLC)等方法测定黑豆、黄豆、绿豆豆芽中异黄酮、多酚的水平,采用1,1-二苯基-2-三硝基苯肼(DPPH)和2,2’-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)方法分析3种豆芽异黄酮和多酚的体外抗氧化活性,研究异黄酮对黑腹果蝇体内超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力的影响。结果表明:黑豆芽的异黄酮、多酚含量最高,黄豆芽次之,绿豆芽最低。4种异黄酮单体中,从黑豆芽中检出染料木苷、大豆苷和黄豆黄苷,从黄豆芽中检出染料木苷和大豆苷,从绿豆芽中只检出大豆苷。3种豆芽异黄酮、多酚提取物的ABTS·+和DPPH·自由基清除能力具有剂量依赖性,拟合方程的相关系数(R2)在0.8957～0.9997区间,提示异黄酮、多酚质量浓度与自由基清除率之间具有较好的量效关系。黑豆芽异黄酮、多酚的IC50值最低,黄豆芽次之,绿豆芽最高,表明黑豆芽的自由基清除能力优于黄豆芽和绿豆芽。3种豆芽异黄酮的IC50值均低于多酚,说明异黄酮的体外抗氧化活性高于多酚。用含有0.5mg/g豆芽异黄酮的培养基饲喂果蝇,可使其SOD、GSH-Px活力明显升高。黑豆芽异黄酮提升果蝇抗氧化酶活力的效果最佳,优于黄豆芽和绿豆芽。本研究为豆芽食品的开发提供了参考依据。Isoflavones and polyphenols in bean sprouts of Glycine max cv. ‘Xiaoliheidou’(black bean), Glycine max(yellow bean) and Vigna radiate(green bean) were determined by various methods such as high-performance liquid chromatography(HPLC). In vitro antioxidant capacities of isoflavones and polyphenols in these three bean sprouts were measured using ABTS [2,2’-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) diammonium salt] and DPPH(1,1-diphenyl-2-picrylhydrazyl) methods. And effects on activities of superoxide dismutase(SOD) and glutathione-peroxidase(GSH-Px) in fruit fly(Drosophila melanogaster) of isoflavones were investigated. Black bean sprouts have the highest contents of isoflavones and polyphenols, followed by yellow bean sprouts and green bean sprouts. Black bean sprouts contain genistin, daidzin and glycitin. Yellow bean sprouts contain genistin and daidzin. And green bean sprouts only contain daidzin. Isoflavone and polyphenol extracts from three bean sprouts scavenge ABTS·+and DPPH· radicals in a dose-dependent manner. And correlation coefficient(R^2) of fitting equation is within the range of 0.8957-0.9997, which indicates the obvious dose-effect relationship between mass concentrations of isoflavones and polyphenols and radical-scavenging capacities. IC50 values of black bean sprouts are the lowest, followed by yellow bean sprouts and green bean sprouts, which shows that radical-scavenging activity of black bean sprouts is higher than those of yellow bean sprouts and green bean sprouts. IC50 values of isoflavones in three bean sprouts are lower than those of polyphenols, which suggests that in vitro antioxidant activity of isoflavones is higher than that of polyphenols. When fruit flies were fed in the medium containing 0.5 mg·g^-1 isoflavone extracts from bean sprouts, SOD and GSH-Px activities increased remarkably.Activities of antioxidant enzymes in fruit flies fed isoflavone extracts from black bean sprouts are higher than those from yellow bean sprouts and green bean sprouts. The present

关 键 词：豆芽 多酚 异黄酮 抗氧化活性 黑腹果蝇 抗氧化酶 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]