啤酒腐败菌的分离鉴定和耐乙酸乳杆菌定量检测方法建立  

Isolation and identification of spoilage bacteria in beer and establishment of quantitative detection method for Lactobacillus acetotolerans

作　　者：栾春光[1] 罗娜[2] 郝建秦[1] 孙亦玄 刘静[2] 陈明[2] 涂京霞[2] 王德良[1] 张五九[1] Chunguang Luan;Na Luo;Yixuan Sun;Jianqin Hao;Jing Liu;Ming Chen;Jingxia TU;Deliang Wang;Wujiu Zhang(China National Research Institute of Food and Fermentation Industries Co.,100095;Guangzhou Zhujiang Brewery Co.,Ltd.)

机构地区：[1]中国食品发酵工业研究院 [2]广州珠江啤酒股份有限公司

出　　处：《中外酒业》2018年第23期14-18,共5页Global Alcinfo

摘　　要：本研究从啤酒污染样品中分离到24株细菌,经鉴定分属11个菌种。利用耐酒花基因horA、horB和horC的特异引物分析表明,不同菌株的耐酒花基因存在差别。研究中首次在啤酒样品中分离到耐乙酸乳杆菌(Lactobacillus acetotolerans )。利用耐酒花基因对耐乙酸乳杆菌的定量检测结果表明,检出限可以达到2.2×10^2CFU/mL。研究结果增加了啤酒腐败菌库中腐败菌的种类,为啤酒生产企业开发针对本企业腐败菌检测提供方法参考。Twenty-four strains of bacteria were isolated from beer samples and identified as 11 species.The specific primers of hop-resistant genes horA,horB and horC were used to analyze the difference of hop-resistant genes among them.Lactobacillus acetotolerans was isolated from beer samples for the first time in this study. Quantitative detection of Lactobacillus acetotolerans by hop-resistant gene showed that the detection limit could reach 2.2×10^2CFU/ml.The research results increase the types of spoilage bacteria in beer spoilage bacteria bank,and provide reference for beer manufacturers to develop detection methods for spoilage bacteria in their own enterprises.

关 键 词：啤酒 腐败菌 耐乙酸乳杆菌 定量检测 

分 类 号：TS262.5[轻工技术与工程—发酵工程;轻工技术与工程—食品科学与工程]