富硒豆豉中安赛蜜、苯甲酸、山梨酸、糖精钠、脱氢乙酸的测定  

Determination of acesulfame potassium, benzoic acid, sorbic acid, saccharin sodium dihydrate and dehydroacetic acid in Se-enriched and fermented soyabeans

作　　者：李碧波 鄢兵华 荣汝繁 LI Bi-Bo;YAN Bing-Hua;RONG Ru-Fan(Xiantao Food and Drug Inspection and Testing Center,Xiantao 433000,China)

机构地区：[1]仙桃市食品药品检验检测中心,仙桃433000

出　　处：《食品安全质量检测学报》2019年第2期521-526,共6页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱法同时测定富硒豆豉中安赛蜜、苯甲酸、山梨酸、糖精钠和脱氢乙酸的分析方法。方法样品经过沉淀剂沉淀蛋白后过滤,使用C_(18)反向色谱柱(pH1.5～9.0)分离,用甲醇-乙酸铵溶液作为流动相进行洗脱,经高效液相色谱紫外检测器和二极管阵列检测器检测,检测波长为230 nm。结果 5种添加剂具有良好的线性关系,相关系数均大于0.9990,检出限为1.0～2.0mg/kg。应用该方法检测富硒豆豉的加标回收率为90.2%～101.1%,相对标准偏差小于2.0%。结论本研究建立的方法有效可行,结果可靠,适用于富硒豆豉中5种添加剂的检测。Objective To establish a method for determination of acesulfame potassium,benzoic acid,sorbic acid,saccharin sodium dihydrate and dehydroacetic acid in Se-enriched and fermented soyabeans by high performance liquid chromatography(HPLC).Methods The sample was deproteinated by precipitator and filtered,then separated on C18 column(pH 1.5-9.0) with methanol and ammonium acetate as mobile phase,then detected by HPLC with UV detector and diode array detector,and the detection wavelength was 230 nm.Results The 5 additives had a good linear relationship,and the correlation coefficients were all greater than 0.9990.The limits of detection were 1.0-2.0 mg/kg.The recoveries of Se-enriched and fermented soybeans were 90.2%-101.1%,with the relative standard deviations of less than 2.0%.Conclusion The established method is feasible and effective,and has the reliable results,which is suitable for the determination of additives in Se-enriched and fermented soyabeans.

关 键 词：安赛蜜 苯甲酸 山梨酸 糖精钠 脱氢乙酸 富硒豆豉 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] TS207.3[理学—化学]