高产谷氨酸菌选育研究进展  

Reserarch Development of High Yielding Glutamic Acid Bacterium Screening and Breeding

作　　者：甄卫军[1] 张石峰[1] 曾宪贤[1] 

机构地区：[1]新疆大学离子束生物工程中心,新疆乌鲁木齐830008

出　　处：《新疆大学学报（自然科学版）》2002年第2期209-211,共3页Journal of Xinjiang University(Natural Science Edition)

基　　金：新疆维吾尔自治区自然科学基金项目 (项目编号 :2 0 0 0 1 );乌鲁木齐市重大科技攻关项目 (项目编号 :Y0 0 1 0 )

摘　　要：对目前谷氨酸菌种选育技术及其发展进行了综述 ,同时重点报道了离子束生物工程技术应用于谷氨酸菌株选育方面的研究 .研究表明 ,以 D1 1 0 、D1 1 0 B为出发菌 ,采用低能 N+ 离子注入技术已经筛选到两株高产菌株D52 2 1 、B32 6 3,其 1 0批次摇瓶平均产酸幅度分别达到 8.4%、7.5 % ,比出发菌分别提高 3 5 .48%和 2 5 % ,摇瓶发酵平均糖酸转化率可以达到 43 % ,RG% (残糖 )达到 2 %以下 。In this paper,the current development and breeding technology of glutamic acid yielding bacterium are summarized. And the study of application of ion beam bioengineering technology to glutamic acid yielding bacterium are emphatically introduced. It is showed that two glutamic acid high yielding strains D\-\{5221\} and B\-\{3263\} are screened out from starting strains of D\-\{110\} and D\-\{110B\} by low energy N\++ ion implantation technology, and the average rocker bottle fermentation GA% can achieve 8.4%?7.5%. They are respectively raised by 35.48%?25%compared to the starting strains. It is concluded that the averge molasses acid transformation of 10 batches rocker bottle fermentation can achieve 43% and RG% is below 2%. Compared to the starting strains, their metabolic capacity are improved.

关 键 词：谷氨酸菌 发酵工业 离子束生物工程 味精 菌种选育 糖酸转化率 低能N 离子注入技术 

分 类 号：TS264.23[轻工技术与工程—发酵工程] TQ922.1[轻工技术与工程—食品科学与工程]