二元酸发酵过程中流加H_2O_2对细胞色素P450酶及产物生成的影响  

Effects of H_2O_2 Addition on Cell Growth and Product Formation inLong-chain Dicarboxylic Acid Fermentation

作　　者：李书良[1] 华玉涛[1] 黄英明[1] 焦鹏[1] 曹竹安[1] 

机构地区：[1]清华大学化工系生物化工研究所,北京100084

出　　处：《微生物学报》2002年第3期359-363,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金重点项目 ( 2 0 0 36 0 1 0 );国家自然科学基金 ( 2 9976 0 2 2 )~~

摘　　要：在二元酸发酵过程中流加H2 O2 对热带假丝酵母发酵生产二元酸有明显的促进作用 ,2mmol L的H2 O2 对产酸的促进作用最为明显 ,比对照提高了 2 6 %。对细胞色素P45 0酶的分析表明 ,流加H2 O2 对细胞色素P45 0酶的活性有明显的促进作用 ,并且细胞色素P45 0酶的活性跟产酸成正相关。此外 ,还进一步分析了流加H2 O2When Candida tropicalis was cultivated in shakig-flask with the H 2O 2 addition, DCA (Dicarboxylic Acid,DCA) concentration was increased, especially at 2mmol/L H 2O 2 concentration. The cytochrome P450 activity assays indicated that H 2O 2 addition significantly increased the activities of cytochrome P450 and DCA production positively correlated with the activities of cytochrome P450. The study on the cell growth demonstrated that the H 2O 2 addition inhibited the cell growth rate. However, the retarding effect was not irreversible since the cell growth rate could recover slowly to the original level after the H 2O 2 addition was halted. The mechanism of inducement on cytochrome P450 by H 2O 2 addition was also discussed in this article.

关 键 词：二元酸 发酵过程 流加H2O2 细胞色素P450酶 产物生成 热带假丝酵母 

分 类 号：TQ920[轻工技术与工程—发酵工程]