生物表面活性剂发酵液的组成及表面活性  

Composition of biosurfactant culture and its surface-active properties

作　　者：李祖义[1] 施邑屏[1] 李江云[1] 徐国梁[1] 潘冰峰[1] 童正新[2] 王芳[2] 

机构地区：[1]中国科学院上海有机化学研究所,上海200032 [2]中国科学院渗流体力学研究所,廊坊102801

出　　处：《工业微生物》2002年第2期1-4,共4页Industrial Microbiology

基　　金：国家 973项目 (G19990 2 2 5 0 1)资助

摘　　要：假单胞菌 (Pseudomonassp .)生长在正烷烃或植物油中 ,能生产出表面活性物质 ,分析其发酵液 ,类脂物和多糖是主要代谢产物。发酵液中表面活性物质主要是糖脂化合物及甘油单脂。发酵液稀释到 5 %,能将表面张力降到 2 7mN/m ,表面性能在广泛 pH(2～ 12 ) ,高矿化度溶液中和高温下都非常稳定。发酵液的良好表面性能显示了它在三次采油、土壤处理等领域中应用潜力。A bacterium, identified as Pseudomonas sp., growing on hydrocarbon or vegetable oil, produced surface- active compounds. The analysis of whole culture broth indicated that the lipids produced were as major metabolite. The surface-active components of the culture broth were mainly glycolipids and monoglyceride. 5%culture broth reduced surface tension to 27mN/m, reduced interfacial tension to 5mN/m and was stable during exposure to high salinity, high temperature and a wide range of pH values, which showed its potential value in enhanced oil-recovery and bioremediation.

关 键 词：生物表面活性剂 发酵液 组成 糖脂 假单胞菌 

分 类 号：TQ920[轻工技术与工程—发酵工程]