气相色谱-质谱法测定藤茶挥发油中化学成分  

Determination of volatile oil components in Ampelopsis grossedentata by GC-MS

作　　者：张世仙[1] 敖克厚[1] 曾启华[1] 刘炎[1] 

机构地区：[1]遵义师范学院化学化工学院黔北特色资源应用研究实验室,贵州遵义563002

出　　处：《中国酿造》2014年第6期140-143,共4页China Brewing

基　　金：贵州省科学技术基金项目(黔科合J字LKZS[2012]28号);遵义师范学院红色文化资源开发与经济研究中心资助项目(ZE2012009);贵州省科技厅资助(黔科合J字[2011]2082号)

摘　　要：采用固相微萃取提取藤茶挥发油,用气相色谱-质谱法分离和鉴定挥发油成分,并用归一化法测定其相对含量,共分离出78个组分,鉴定出63种化合物,其含量占挥发油组分的80.6%。藤茶挥发油主要成分:乙醇16.66%、1,3-二叔丁基苯9.44%、2-甲基癸烷4.95%、2,4-二甲基-1-癸烯4.65%、2,4-二甲基-1-庚烯4.51%、7-甲基-十一烯4.45%、2,6-二甲基壬烷4.36%、桧烯1.86%、α-蒎烯1.85%、甲酸乙酯1.83%、十三烷1.72%、β-崖柏酮1.44%、1-十一烯1.29%、2,3,5,8-四甲基-十一烷1.27%、4,6-二甲基-十二烷1.02%等,占挥发性化合物总量的76%。Volatile oil in Ampelopsis grossedentata were extracted by solid phase micro-extraction, separated and identified by GC-MS, and the relative content was determined by normalization method. 78 components were isolated and 63 compounds were identified, which accounted for 80.6% of volatile oil components. Result showed that the main components of volatile oil in A. grossedentata were： ethanol（16.66%）, 1.3-di-tert-butyl benzene（9.44%）, 2-methyl decane（4.95%）, 2.4-dimethyl-1-decene（4.65%）, 2.4-dimethyl-1-heptene（4.51%）, 7-methy-11-undecene（4.45%）,2.6-dimethyl-nonane（4.36%）, sabinene（1.86%）, α-pinene（1.85%）, ethylformate（1.83%）, tridecane（1.72%）, β-thujone（1.44%）, 1-undecene（1.29%）, 2,3,5,8-tetramethyl-decane（1.27%）, 4.6-dimethyl-dodecane（1.02%）, accounting for 76% of the total volatile compounds.

关 键 词：气相色谱-质谱 挥发油 藤茶 

分 类 号：Q539[生物学—生物化学]