刺参多糖中糖醛酸、氨基糖和中性单糖的同步测定方法研究  

Simultaneous determination of uronic acids,amino sugars and neutral sugars in polysaccharide from sea cucumber

作　　者：田鑫[1] 盛文静[1] 赵园园[1] 董军[1] 薛勇[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品工业科技》2014年第15期85-89,94,共6页Science and Technology of Food Industry

基　　金：泰山学者建设工程专项经费;海洋公益性行业科研专项(201105029)

摘　　要：建立柱前1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生高效液相色谱法(HPLC)测定刺参多糖中糖醛酸、氨基糖和中性单糖的方法。刺参多糖用2mol/L三氟乙酸水解、PMP衍生化后,用反相HPLC检测,实现了糖醛酸,氨基糖和中性单糖的良好分离和测定。测定结果表明,刺参多糖含有甘露糖、半乳糖、岩藻糖、氨基葡萄糖、氨基半乳糖和葡萄糖醛酸6种单糖。该方法简便快捷,灵敏度高,重现性良好。A high performance liquid chromatographic （HPLC）method for simultaneous determination of uronic acids,amino sugars and neutral sugars in polysaccharide from sea cucumber by pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone （PMP） was developed. The polysaccharide was hydrolyzed by the 2mol/L trifluoroacetic acid, and then derivatived with PMP. The uronic acids, amino sugars and neutral sugars were separated well by reverse-phase HPLC. The results showed that glycosaminoglycan consisted of mannose, galactose, fucose, glucosamine, galactosamine, glucuronic acid.The HPLC method is simple, rapid, sensitive, and convenient.

关 键 词：刺参 多糖 高效液相色谱 柱前衍生 1-苯基-3-甲基-5-吡唑啉酮 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]