进口水产品中副溶血性弧菌耐药性和毒力基因的调查  

Survey of drug resistance and virulence gene of Vibrio parahaemolyticus from imported aquatic products

作　　者：于纪棉[1] 倪晶晶[1] 陶冬英[1] 劳华均[2] 王建峰[2] 王春[2] 

机构地区：[1]宁波卫生职业技术学院,浙江宁波315100 [2]宁波出入境检验检疫局,浙江宁波315012

出　　处：《中国食品卫生杂志》2014年第4期320-323,共4页Chinese Journal of Food Hygiene

基　　金：宁波市自然科学基金(2012A610180);宁波卫生职业技术学院自然基金(201101)

摘　　要：目的了解宁波口岸进口水产品中副溶血性弧菌的耐药性和毒力基因分布情况。方法将分离自宁波口岸进口水产品中的129株副溶血性弧菌作为研究对象,利用K-B纸片扩散法测定细菌对15种抗生素的耐药性,使用PCR方法检测副溶血性弧菌的4个毒力基因(tdh、tlh、trh、toxR)。结果菌株对氨苄西林、羧苄西林、头孢拉定的耐药率分别为90.70%、98.45%和51.17%;对卡那霉素、头孢氨苄、头孢唑啉、头孢呋辛的耐药率分别为1.55%、13.18%、2.33%、20.16%,中敏感率分别为33.33%、52.71%、46.51%、40.31%;其他8种抗生素(四环素、强力霉素、阿米卡星、庆大霉素、头孢哌酮、氧氟沙星、氯霉素、复方新诺明)则比较敏感;毒力基因trh,tdh,tlh和toxR的携带率分别为0%、0.78%、100%和100%。结论强力霉素、庆大霉素、复方新诺明是治疗由进口水产品引起的副溶血性弧菌感染的首选抗生素;进口水产品中副溶血性弧菌的主要致病毒力基因携带率比较低。Objective To survey the drug resistance and the distribution of virulence gene in isolated Vibrio parahaemolyticus （VP） from the imported aquatic products at Ningbo port. Methods The 129 VP strains were isolated from imported aquatic products at Ningbo port and 15 antibiotics were used for drug resistance test by K-B test method. Polymerase chain reaction was used for testing the 4 virulence genes （tlh, trh, tdh, toxR） from VP. Results The drug resistance rates of ampicillin, carbenicillin and cefradine were 90. 70% , 98.45% and 51. 17% , respectively. The drug resistance rates of kanamycin, cefalexin, cephazoline and cefuroximc were 1.55% , 13.18% , 2.33% and 20. 16% , respectively. Medium sensitive rates of kanamycin, cefalexin, cephazoline and cefuroxime were 33.33%, 52.71%, 46.51% , 40.31% , respectively. All strains were sensitive to the 8 antibiotics （ Tetracycline, doxycycline, amikacin, gentamicin, cefoperazone, ofloxacin, chloramphenicol, co-trimoxazole）. The detection rates of trh, tdh, tlh and toxR genes were 0% , 0.78% , 100% and 100% , respectively. Conclusion The doxycycline, gentamicin, co-trimoxazole were the preferred antibiotic for people infected by VP from the imported aquatic products. The rate of VP strains carrying main virulence gene was relatively low in VP strains in the imported aquatic products.

关 键 词：副溶血性弧菌 耐药 毒力基因 食源性致病菌 抗生素 水产品 药敏试验 

分 类 号：R155.5[医药卫生—营养与食品卫生学]