单环刺螠糖胺聚糖组成结构与功能活性研究  

The Study on Composition Structure and Functional Activity of Glycosaminoglycan from Urechis unicinctus

作　　者：袁春营[1] 刘萍[1] 韩旭[1] 崔青曼[1] 

机构地区：[1]天津市海洋资源与化学重点实验室,天津科技大学海洋科学与工程学院,天津300457

出　　处：《食品工业》2014年第8期181-183,共3页The Food Industry

基　　金：天津市科技兴海项目(KX2010-0003)

摘　　要：研究了单环刺螠糖胺聚糖的化学组成、官能团及抗氧化和抗凝血活性,结果表明:糖胺聚糖的硫酸根含量均值为30.26%,糖醛酸含量均值为25.25%,氨基糖含量均值为7.58%;糖胺聚糖中含有-OH(3 418 cm-1)、-COO-(1 642 cm-1)、-SO2(1 415 cm-1)、C-O-C(糖环,1 034 cm-1)等特征基团;在0.1 mg/mL^0.5 mg/mL质量浓度范围内,糖胺聚糖对羟基自由基的清除作用较强,清除率在80%以上,清除超氧阴离子自由基的能力较弱;体外试验证实该糖胺聚糖可明显延长大耳白家兔的PT和APTT,对TT的影响较小,可能存在着不同于肝素的抗凝血途径。The chemical composition, functional group, antioxidant and anticoagulant activity of glycosaminoglycan from Urechis unicinctus were studied, the results showed that： the average content of sulfate, uronic acid and amino sugar were 30.26%, 25.25% and 7.58%, respectively; glycosaminoglycan contained characteristic groups such as -OH （3 418 cm-1）, -COO-（1 642 cm-1）, -SO2（1 415 cm-1） and C-O-C （saccharide ring, 1 034 cm-1） ere; within the range of 0.1-0.5 mg/mL, glycosaminoglycan had a strong scavenging effect on hydroxyl radicals, and scavenging rate was above 80%, but had a weak scavenging effect on superoxide anion radical; glycosaminoglycan could obviously extend the PT and AFFF of big ears rabbits, slightly prolonged TT, there may be a anticoagulant way different from the heparin.

关 键 词：单环刺螠 糖胺聚糖 化学组成 红外光谱 功能活性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]