响应面法优化米曲霉产孢工艺条件  

Optimization of Aspergillus oryzae spores-producing process condition by response surface analysis

作　　者：张义伟[1] 刘瑞勇[1] 李新锐[1] 刘旭博[1] 张良[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,辽宁沈阳110866

出　　处：《中国酿造》2014年第7期34-38,共5页China Brewing

基　　金：国家科技部"十二五"科技支撑计划项目(2012BAD31B05-3);沈阳农业大学大学生科技创新项目(201310157539)

摘　　要：米曲霉(Aspergillusoryzae)是一种在食品中应用广泛的真菌。为了得到较多的米曲霉孢子,基于单因素试验选取了培养温度、培养基含水量和接种量3个因素,利用Design-Expert响应面分析法对产孢进行了优化。结果表明,在培养温度为22.66℃,培养基含水量为48.79%,接种量为3.44‰时,产孢子数预测可达到12.7437×109CFU/g,实际测得为12.1×109CFU/g,达到预测值的94.95%。A spergilllus oryzae isw idely used in food.In order to getm orc spores of A.oryzae,based on single factor test,three factors of culture temperature,m edium water contentand inoculum s were selected to conductresponse surface analysis to optim ize spores production from A.oryzae.The results show ed thatunder the condition of tem perature 22.66 ℃,m edium w ater content48.79％,inoculum 3.44‰,the predicted spores were up to 12.743 7×109 C FU/g,and the actualdetected num berw as 12.1× 109 C FU/g,maching 94.95％ of the predicted value.

关 键 词：米曲霉 孢子粉 响应面优化 

分 类 号：Q815[生物学—生物工程]