木糖葡萄球菌高密度培养条件的研究  

High-density Culture Conditions of Staphylococcus xylosus

作　　者：贾娜[1,2] 马宏慧[3] 韩齐[3] 孔保华[3] 夏秀芳[3] 刘骞[3] 

机构地区：[1]渤海大学食品科学研究院 [2]渤海大学化学化工与食品安全学院辽宁省食品安全重点实验室食品贮藏加工及质量安全控制工程技术研究中心辽宁省高校重大科技平台,辽宁锦州121013 [3]东北农业大学食品学院,哈尔滨150030

出　　处：《中国食品学报》2014年第7期130-137,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31271897);公益性行业(农业)科研专项(201303082);哈尔滨市科技创新人才研究专项(2012RFXXN105)

摘　　要：采用正交试验优化木糖葡萄球菌A2的增殖培养基配方,并确定其高密度培养条件和高密度培养物的富集条件。通过单因素试验确定木糖葡萄球菌A2液体培养基的最佳碳源为乳糖,最佳氮源为示蛋白胨,最佳营养因子为酵母膏和香菇汁;利用L9(34)正交试验得出增殖培养基的最佳配方为0.5%乳糖、2%示蛋白胨、0.2%酵母膏、5%香菇汁和7.5%NaCl。木糖葡萄球菌A2高密度培养的最适初始pH值为7.5,最适装液量为90%。以最佳培养基和培养条件在32℃培养17 h后,菌液中活菌数可达到3.43×109CFU/mL。对高密度培养物进行富集的最佳条件为6 000 r/min离心30 min,离心后菌种存活率为82.5%,浓缩倍数为74.48倍,活菌菌密度可达2.07×1011CFU/g。本研究成果可为生产发酵肉制品的直投式发酵剂提供优良菌种。Orthogonal experiment was conducted to optimize the enrichment media formula of Staphylococcus xylosus.The high-density culture conditions of Staphylococcus xylosus and the concentration parameters of high-density cultures were also determined. The single factor experiment results showed that the optimal carbon source and nitrogen source were lactose and proteose peptone, respectively; and the optimal nutritional factor were yeast extract paste and mushroom juice. The L9（34） orthogonal experiments results showed that the optimal formula of the enrichment media was consist of0.5% lactose, 2% proteose peptone, 0.2% yeast extract paste, 5% mushroom juice and 7.5% NaCl. The optimal initial pH for the high-density culture of Staphylococcus xylosus A2 was 7.5 and the optimal liquid volume in flask was 90%.Under the optimal conditions, the maximum viable counts of the strain reached 3.43×109CFU/mL after cultured at 37 ℃for 12 h. After centrifugalized at 4 000 r/min for 30 min, the maximum starter survival rate of 82.5% was observed, and there were 74.48-fold of enrichment. The finally viable count was 2.07 ×1011CFU/g. The present research could supply ready-to-use starter for the production of fermented meat products.

关 键 词：木糖葡萄球菌 培养基配方 高密度培养 富集 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]