多倍体啤酒酵母URA3基因的失活与恢复  

Inactivation and Recovery of URA3 Gene of Polyploid Brewer's Yeast

作　　者：许海艳[1] 赵丽彬[1] 董健[1] 肖冬光[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《酿酒科技》2014年第8期24-26,共3页Liquor-Making Science & Technology

基　　金：食品微生物基因组改造(国家863计划)(SS2012AA022108);酿酒原料高效安全制造技术研究("十二五"农村领域国家科技计划课题子课题;2013AA102108);天津科技大学校基金项目(20120114)

摘　　要：以多倍体啤酒酵母菌株S6作为出发菌株,利用同源重组的方法构建了尿嘧啶营养缺陷型啤酒酵母菌株S6-ΔU,使之用于遗传标记,并把缺陷型菌株恢复成原营养型S6'。同时对出发菌株S6,缺陷型菌株S6-ΔU和恢复后的菌株S6'的生长状况进行比较。结果表明,URA3点突变并没有弱化菌株的生长状况。从而建立一种快速获得缺陷型啤酒酵母菌株的方法。Polyploid brewer＇s yeast strain S6 was used as the starting strain to construct uracil auxotrophic strain S6-△U through gene homologous recombination, which could be used as a genetic marker. Then uracil auxotrophic strain S6-△U was restored to original strain and named strain S6＇. The growth of strain S6, strain S6-△U and strain S6＇ was compared and the results suggested that the mutation of URA3 gene would not re-duce the growth of the strain. Accordingly, a method for rapid preparation of auxotrophic brewer＇s strains had been established in this study.

关 键 词：微生物 啤酒酵母 多倍体 URA3 失活 恢复 啤酒 URA 3 

分 类 号：TS262.5[轻工技术与工程—发酵工程]