牧区奶干渣组合式发酵剂响应面法优化  

Optimization of Combined Fermentation Starter of Milk Dried Residue in Pastoral Areas by Response Surface Methodology

作　　者：文鹏程[1] 王军[1] 任发政[2] 韩玲[1] 王琳琳[1] 纪银莉 

机构地区：[1]甘肃农业大学食品科学与工程学院,兰州730070 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]甘肃华羚生物技术研究中心,兰州730000

出　　处：《农业机械学报》2014年第8期241-247,共7页Transactions of the Chinese Society for Agricultural Machinery

基　　金：公益性行业(农业)科研专项经费资助项目(201303085);国家国际科技合作专项资助项目(2011DFA32550)

摘　　要：以脱脂牦牛乳为试验材料,发酵3.5 h滴定酸度为试验指标,在不同菌种最佳组合方式筛选的研究基础上,利用响应面Box-Behnken中心组合试验研究了4株筛自牧区、发酵性能优良的菌种:保加利亚乳杆菌(MGD1-3)、嗜热链球菌(MGB39-5)、嗜热链球菌(G81-1)、植物乳杆菌(BM5152)不同接种比例对牧区奶干渣发酵效果的影响。结果表明,优化得到最优组合式发酵剂为:MGD1-3体积分数3.06%、MGB39-5体积分数0.64%、G81-1体积分数0.55%、BM5152体积分数2.04%,滴定酸度为75.87°T。为了便于实际操作,确定最终体积接种比为:MGD1-3∶MGB39-5∶G81-1∶BM5152=30∶6∶5∶20,接种量体积分数为6.0%,该条件下进行3次平行试验,得到滴定酸度平均值为74.21°T;将接种优化后组合式发酵剂制成的奶干渣(试验组)与牧区采集奶干渣(对照组)进行感官与理化指标比较,结果表明该组合式发酵剂发酵性能优良。Skimmed yak milk was selected for this research and the titratable acidity at 3.5 h as the test indicator. Box- Behnken central composite experiment was designed based on optimal combination of fermentation starters to research the effect of Lactobacillus delbrueckii subsp bulgaricus （ MGD1 - 3 ） , Streptococcus thermophilus （ MGB39 - 5 ）, Streptococcus thermophilus （ G81 - 1 ）, and Lactobacillus plantarum（BM5152）, which were screened from pastoral areas to the titratable acidity. The result showed that the optimal combination of fermentation starters was MGD1 -3 3.06% , MGB39 -5 0. 64% , G81 - 1 0.55% , and BM5152 2.04% and the titratable acidity was 75.87~% In order to facilitate the practical operation, the proportion of MGD1 -3：MGB39 -5：GS1 - 1：BM5152 = 30： 6：5：20 and 6.0% of inoculum size based on volume fraction were selected. Under these conditions, the average titratable acidity was 74.21°T of three parallel trials. Compared with the sensory and physical and chemical indicators of milk dried residue came from optimized combination of fermentation starters and pastoral areas, the result showed that the optimized combination of fermentation starters was reliable.

关 键 词：奶干渣 乳酸菌 响应面 滴定酸度 

分 类 号：TS252.4[轻工技术与工程—农产品加工及贮藏工程]