超微粉碎辅助超声-微波法提取玉米黄色素  

Ultrafine Comminution-assisted Ultrasonic-microwave Synergistic Extraction of Maize Yellow Pigment

作　　者：李晓玲[1,2] 陈相艳[2] 王文亮[2] 徐同成[2] 王世清[1] 石贤权[2] 

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]山东省农业科学院农产品研究所,济南250100

出　　处：《中国食品学报》2014年第8期99-107,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31271963);山东省农业重大应用技术创新课题(鲁财农指[2011]39)

摘　　要：为研究从玉米蛋白粉中提取玉米黄色素的最佳方法,首次采用超微粉碎技术处理原料玉米蛋白粉。在完全随机单因素设计的基础上,选取微粉粒度、超声功率、微波功率和微波时间为自变量,玉米黄色素提取率为响应值,采用Box-Behnken中心组合设计,在微波-超声组合反应系统中强化提取玉米蛋白粉中的黄色素。试验结果表明,参试因子贡献大小为:微粉粒度>微波功率>微波时间>超声功率。经响应面优化的最优工艺是:微粉粒度500目、微波功率480 W、微波时间115 s、超声功率480 W。在该试验条件下玉米黄色素得率为0.640 mg/g,表明超微粉碎和超声-微波组合协同辅助方法可显著提高玉米黄色素的提取率(P<0.001)。对样品的液相色谱分析表明,提取纯化所得样品纯度为86.64%,其中叶黄素的含量约为67.91%。To optimize the extraction conditions of maize yellow pigment from maize protein powder, ultrafine comminution was firstly applied to material pretreatment. Based on single factor experiments, and extraction ratio as response values, powder size, ultrasonic power, microwave power and microwave time as response factors, extraction of maize yellow pigment from maize protein powder was conducted using Box-Behnken central composite experimental design. Results showed contribution of the factors in the tests were： powder size〉microwave power〉microwave time〉ultrasonic power. Through single factor experiments, we found that powder size and the ultrasonic power had significant effects on the per- formance during the whole extraction process, while the ultrasonic time was dispensable. The optimum conditions of ex- traction obtained by response surface methodology were as followed： maize protein powder at 500 mesh, ultrasonic power of 480W, microwave power of 480W for 115 s. Under these conditions, the extraction ratio was 0.640 mg/g. The model indicated that uhrafine comminution and ultrasonic-microwave synergistic extraction have a significant impact on extraction ratio of maize yellow pigment. HPLC analysis showed that purity of the sample was 86.64%. The content of hydroxyl- carotenoids was 67.91%.

关 键 词：玉米黄色素 超微粉碎 响应面 协同萃取 

分 类 号：TS264.4[轻工技术与工程—发酵工程]